biostudies-literatureChiti MCWallonie-Bruxelles InternationalDonations from Ferrero familyFonds National de la Recherche Scientifique de Belgique (FNRS)Foundation Against CancerFonds Spéciaux de Recherche, Fondation St Luc71https://www.ebi.ac.uk/biostudies/studies/S-EPMC5654051biostudies-literatureUnknown10(1)<h4>Background</h4>Ovarian tissue cryopreservation followed by transplantation after cancer remission is the most commonly applied fertility restoration approach in very young girls and women who require immediate cancer therapy. However, clinicians strongly advise against reimplantation of one's own ovarian tissue when there is a high risk of recurrence after grafting. For these patients, development of an alternative strategy, namely a transplantable artificial ovary, offers future hope of conceiving. The first essential requirement for an artificial ovary is the set-up of a safe and effective follicle isolation procedure. Despite encouraging results with different variants of this technique, none of them take into the account the physiology and great variability in follicular density inJournal of ovarian researchA modified and tailored human follicle isolation procedure improves follicle recovery and survival.PMC5654051grant 5/4/150/5Cernogoraz AHobeika MChiti MCDolmans MMDonnez JAmorim CAfalseA modified and tailored human follicle isolation procedure improves follicle recovery and survival.<h4>Background</h4>Ovarian tissue cryopreservation followed by transplantation after cancer remission is the most commonly applied fertility restoration approach in very young girls and women who require immediate cancer therapy. However, clinicians strongly advise against reimplantation of one's own ovarian tissue when there is a high risk of recurrence after grafting. For these patients, development of an alternative strategy, namely a transplantable artificial ovary, offers future hope of conceiving. The first essential requirement for an artificial ovary is the set-up of a safe and effective follicle isolation procedure. Despite encouraging results with different variants of this technique, none of them take into the account the physiology and great variability in follicular density in2017-01-01T00:00:00Z2017 Oct2025-04-03T22:41:48.248Z2019-03-27T02:59:45ZS-EPMC56540512906114910.1186/s13048-017-0366-8