{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Liu Z"],"pubmed_abstract":["Systemic Lupus Erythematosus (SLE) and pemphigus are two representative autoimmune diseases driven by pathogenic autoantibody systemically and organ-specifically, respectively. Given the involvement of antibody in the pathogenesis, B cells are inclined to differentiate and function in an abnormal activation model. Here we defined a unique CD19<sup>hi</sup> B cell population existing in the periphery of SLE and pemphigus patients as well as in human tonsils. CD19<sup>hi</sup> B cells could be induced in vitro after co-culturing fully activated CD4<sup>+</sup> T cells with autologous B cells. They expressed high levels of HLA-DR, IgG, IgM and multiple ligands of costimulatory molecules with the capacity to produce extra IgG and IgM. Transcirptome assay revealed that genes involved in B-cell activation and differentiation were up-regulated in CD19<sup>hi</sup> B cells. Antibody blockade experiments showed that the interactions between costimulatory molecules contributed to CD19<sup>hi</sup> B-cell generation and IgG/IgM production. What is more, frequencies of peripheral CD19<sup>hi</sup> B cells from SLE and pemphigus patients were correlated with serum total IgG and IgM, but not with autoantigen-specific antibodies and disease severity. Therefore, our investigation demonstrates that CD19<sup>hi</sup> B cells might contain B cell precursors for terminal differentiation and contribute to total IgG/IgM production in human autoimmune diseases."],"journal":["Scientific reports"],"pagination":["13921"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5655037"],"repository":["biostudies-literature"],"pubmed_title":["Peripheral CD19<sup>hi</sup> B cells exhibit activated phenotype and functionality in promoting IgG and IgM production in human autoimmune diseases."],"pmcid":["PMC5655037"],"pubmed_authors":["Zheng J","Liu Z","Wang S","Huang X","Wang Y","Zeng W","Pan M"],"additional_accession":[]},"is_claimable":false,"name":"Peripheral CD19<sup>hi</sup> B cells exhibit activated phenotype and functionality in promoting IgG and IgM production in human autoimmune diseases.","description":"Systemic Lupus Erythematosus (SLE) and pemphigus are two representative autoimmune diseases driven by pathogenic autoantibody systemically and organ-specifically, respectively. Given the involvement of antibody in the pathogenesis, B cells are inclined to differentiate and function in an abnormal activation model. Here we defined a unique CD19<sup>hi</sup> B cell population existing in the periphery of SLE and pemphigus patients as well as in human tonsils. CD19<sup>hi</sup> B cells could be induced in vitro after co-culturing fully activated CD4<sup>+</sup> T cells with autologous B cells. They expressed high levels of HLA-DR, IgG, IgM and multiple ligands of costimulatory molecules with the capacity to produce extra IgG and IgM. Transcirptome assay revealed that genes involved in B-cell activation and differentiation were up-regulated in CD19<sup>hi</sup> B cells. Antibody blockade experiments showed that the interactions between costimulatory molecules contributed to CD19<sup>hi</sup> B-cell generation and IgG/IgM production. What is more, frequencies of peripheral CD19<sup>hi</sup> B cells from SLE and pemphigus patients were correlated with serum total IgG and IgM, but not with autoantigen-specific antibodies and disease severity. Therefore, our investigation demonstrates that CD19<sup>hi</sup> B cells might contain B cell precursors for terminal differentiation and contribute to total IgG/IgM production in human autoimmune diseases.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Oct","modification":"2026-05-03T08:02:36.01Z","creation":"2019-03-27T02:59:47Z"},"accession":"S-EPMC5655037","cross_references":{"pubmed":["29066741"],"doi":["10.1038/s41598-017-14089-2"]}}