{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moorthy GS"],"funding":["NHLBI NIH HHS","Impact of Hypothermia on Midazolam/Morphine Pharmacokinetics","Impact of Pharmacology on Duration of Ventilation in Patients with Respiratory Failure"],"pagination":["1-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5662017"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1067"],"pubmed_abstract":["Pharmacokinetic, pharmacodynamic and pharmacogenomic studies of midazolam are currently being performed in critically ill children to find suitable dose regimens. Sensitive assays using small volumes of plasma are necessary to determine the concentrations of midazolam and its respective metabolites in pediatric studies. Midazolam is metabolized to hydroxylated midazolam isomers, which are present as free as well as the corresponding glucuronide conjugates. A high-performance liquid chromatographic method with tandem mass spectrometry has been developed and validated for the quantification of midazolam, and free and total 1-hydroxymidazolam and 4-hydroxymidazolam metabolites in small volumes of plasma. Cleanup consisted of 96-well μ-elution solid phase extraction (SPE). The analytes were se"],"journal":["Journal of chromatography. B, Analytical technologies in the biomedical and life sciences"],"pubmed_title":["Development and validation of a sensitive assay for analysis of midazolam, free and conjugated 1-hydroxymidazolam and 4-hydroxymidazolam in pediatric plasma: Application to Pediatric Pharmacokinetic Study."],"pmcid":["PMC5662017"],"funding_grant_id":["5R01HL112745","R01 HL098087","5R01HL098087","R01 HL112745"],"pubmed_authors":["Moorthy GS","Jogiraju H","Zuppa AF","Vedar C"],"additional_accession":[]},"is_claimable":false,"name":"Development and validation of a sensitive assay for analysis of midazolam, free and conjugated 1-hydroxymidazolam and 4-hydroxymidazolam in pediatric plasma: Application to Pediatric Pharmacokinetic Study.","description":"Pharmacokinetic, pharmacodynamic and pharmacogenomic studies of midazolam are currently being performed in critically ill children to find suitable dose regimens. Sensitive assays using small volumes of plasma are necessary to determine the concentrations of midazolam and its respective metabolites in pediatric studies. Midazolam is metabolized to hydroxylated midazolam isomers, which are present as free as well as the corresponding glucuronide conjugates. A high-performance liquid chromatographic method with tandem mass spectrometry has been developed and validated for the quantification of midazolam, and free and total 1-hydroxymidazolam and 4-hydroxymidazolam metabolites in small volumes of plasma. Cleanup consisted of 96-well μ-elution solid phase extraction (SPE). The analytes were se","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-04-30T01:01:22.975Z","creation":"2019-03-27T00:04:42Z"},"accession":"S-EPMC5662017","cross_references":{"pubmed":["28978489"],"doi":["10.1016/j.jchromb.2017.09.030"]}}