{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jobe O"],"funding":["Henry M. Jackson Foundation","NCATS NIH HHS","NCI NIH HHS"],"pagination":["1352"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5662875"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8"],"pubmed_abstract":["Macrophages are a major target for human immunodeficiency virus type 1 (HIV-1) infection. However, macrophages are largely heterogeneous and may exhibit differences in permissiveness to HIV-1 infection. This study highlights the interplay of macrophage heterogeneity in HIV-1 pathogenesis. We show that monocyte-derived macrophages (MDMs) could be divided into two distinct subsets: CD14<sup>+</sup>Siglec-1<sup>hi</sup>CD4<sup>+</sup> (non-adherent MDM) and CD14<sup>+</sup>Siglec-1<sup>Lo</sup>CD4<sup>-</sup> (adherent MDM). The CD14<sup>+</sup>Siglec-1<sup>hi</sup>CD4<sup>+</sup>MDM subset represented the smaller proportion in the macrophage pool, and varied among different donors. Fractionation and subsequent exposure of the two MDM subsets to HIV-1 revealed opposite outcomes in terms of HI"],"journal":["Frontiers in immunology"],"pubmed_title":["Human Primary Macrophages Derived <i>In Vitro</i> from Circulating Monocytes Comprise Adherent and Non-Adherent Subsets with Differential Expression of Siglec-1 and CD4 and Permissiveness to HIV-1 Infection."],"pmcid":["PMC5662875"],"funding_grant_id":["P30 CA091842","UL1 TR002345","W81XWH-11-2-0174","UL1 TR000448"],"pubmed_authors":["Alving CR","Tycksen E","Onkar S","Jobe O","Kim J","Michael NL","Rao M"],"additional_accession":[]},"is_claimable":false,"name":"Human Primary Macrophages Derived <i>In Vitro</i> from Circulating Monocytes Comprise Adherent and Non-Adherent Subsets with Differential Expression of Siglec-1 and CD4 and Permissiveness to HIV-1 Infection.","description":"Macrophages are a major target for human immunodeficiency virus type 1 (HIV-1) infection. However, macrophages are largely heterogeneous and may exhibit differences in permissiveness to HIV-1 infection. This study highlights the interplay of macrophage heterogeneity in HIV-1 pathogenesis. We show that monocyte-derived macrophages (MDMs) could be divided into two distinct subsets: CD14<sup>+</sup>Siglec-1<sup>hi</sup>CD4<sup>+</sup> (non-adherent MDM) and CD14<sup>+</sup>Siglec-1<sup>Lo</sup>CD4<sup>-</sup> (adherent MDM). The CD14<sup>+</sup>Siglec-1<sup>hi</sup>CD4<sup>+</sup>MDM subset represented the smaller proportion in the macrophage pool, and varied among different donors. Fractionation and subsequent exposure of the two MDM subsets to HIV-1 revealed opposite outcomes in terms of HI","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-04-07T17:02:29.729Z","creation":"2019-03-27T03:00:23Z"},"accession":"S-EPMC5662875","cross_references":{"pubmed":["29123518"],"doi":["10.3389/fimmu.2017.01352"]}}