biostudies-literatureJobe OHenry M. Jackson FoundationNCATS NIH HHSNCI NIH HHS1352https://www.ebi.ac.uk/biostudies/studies/S-EPMC5662875biostudies-literatureUnknown8Macrophages are a major target for human immunodeficiency virus type 1 (HIV-1) infection. However, macrophages are largely heterogeneous and may exhibit differences in permissiveness to HIV-1 infection. This study highlights the interplay of macrophage heterogeneity in HIV-1 pathogenesis. We show that monocyte-derived macrophages (MDMs) could be divided into two distinct subsets: CD14⁺Siglec-1^hiCD4⁺ (non-adherent MDM) and CD14⁺Siglec-1^LoCD4^- (adherent MDM). The CD14⁺Siglec-1^hiCD4⁺MDM subset represented the smaller proportion in the macrophage pool, and varied among different donors. Fractionation and subsequent exposure of the two MDM subsets to HIV-1 revealed opposite outcomes in terms of HIFrontiers in immunologyHuman Primary Macrophages Derived <i>In Vitro</i> from Circulating Monocytes Comprise Adherent and Non-Adherent Subsets with Differential Expression of Siglec-1 and CD4 and Permissiveness to HIV-1 Infection.PMC5662875P30 CA091842UL1 TR002345W81XWH-11-2-0174UL1 TR000448Alving CRTycksen EOnkar SJobe OKim JMichael NLRao MfalseHuman Primary Macrophages Derived <i>In Vitro</i> from Circulating Monocytes Comprise Adherent and Non-Adherent Subsets with Differential Expression of Siglec-1 and CD4 and Permissiveness to HIV-1 Infection.Macrophages are a major target for human immunodeficiency virus type 1 (HIV-1) infection. However, macrophages are largely heterogeneous and may exhibit differences in permissiveness to HIV-1 infection. This study highlights the interplay of macrophage heterogeneity in HIV-1 pathogenesis. We show that monocyte-derived macrophages (MDMs) could be divided into two distinct subsets: CD14⁺Siglec-1^hiCD4⁺ (non-adherent MDM) and CD14⁺Siglec-1^LoCD4^- (adherent MDM). The CD14⁺Siglec-1^hiCD4⁺MDM subset represented the smaller proportion in the macrophage pool, and varied among different donors. Fractionation and subsequent exposure of the two MDM subsets to HIV-1 revealed opposite outcomes in terms of HI2017-01-01T00:00:00Z20172026-04-07T17:02:29.729Z2019-03-27T03:00:23ZS-EPMC56628752912351810.3389/fimmu.2017.01352