biostudies-literatureUnknown7(1)Carnevale GIntrahepatic cholangiocarcinoma (iCCA) represents a heterogeneous group of malignancies emerging from the biliary tree, often in the context of chronic bile ducts inflammation. The immunological features of iCCA cells and their capability to control the lymphocytes response have not yet been investigated. The aims of the present study were to evaluate the interaction between iCCA cells and human peripheral blood mononuclear cells (PBMCs) and the role of Fas/FasL in modulating T-cells and NK-cells response after direct co-culture. iCCA cells express high levels of Fas and FasL that increase after co-culture with PBMCs inducing apoptosis in CD4⁺, CD8⁺ T-cells and in CD56⁺ NK-cells. In vitro, c-FLIP is expressed in iCCA cells and the co-culture with PBMCs induScientific reports14419https://www.ebi.ac.uk/biostudies/studies/S-EPMC5663931biostudies-literatureActivation of Fas/FasL pathway and the role of c-FLIP in primary culture of human cholangiocarcinoma cells.PMC5663931Alvaro DRiccio MPisciotta ACarpino GCossarizza Ade Pol ACardinale VCarnevale GBertoni LOveri DGibellini LNevi LDe Biasi SCostantini DGaudio EfalseActivation of Fas/FasL pathway and the role of c-FLIP in primary culture of human cholangiocarcinoma cells.Intrahepatic cholangiocarcinoma (iCCA) represents a heterogeneous group of malignancies emerging from the biliary tree, often in the context of chronic bile ducts inflammation. The immunological features of iCCA cells and their capability to control the lymphocytes response have not yet been investigated. The aims of the present study were to evaluate the interaction between iCCA cells and human peripheral blood mononuclear cells (PBMCs) and the role of Fas/FasL in modulating T-cells and NK-cells response after direct co-culture. iCCA cells express high levels of Fas and FasL that increase after co-culture with PBMCs inducing apoptosis in CD4⁺, CD8⁺ T-cells and in CD56⁺ NK-cells. In vitro, c-FLIP is expressed in iCCA cells and the co-culture with PBMCs indu2017-01-01T00:00:00Z2017 Oct2026-05-05T23:28:02.248Z2019-03-27T03:00:28ZS-EPMC56639312908954510.1038/s41598-017-14838-3