biostudies-literatureJardine KJU.S. Department of EnergyE2045https://www.ebi.ac.uk/biostudies/studies/S-EPMC5666727biostudies-literatureUnknown18(10)C₁ metabolism in plants is known to be involved in photorespiration, nitrogen and amino acid metabolism, as well as methylation and biosynthesis of metabolites and biopolymers. Although the flux of carbon through the C₁ pathway is thought to be large, its intermediates are difficult to measure and relatively little is known about this potentially ubiquitous pathway. In this study, we evaluated the C₁ pathway and its integration with the central metabolism using aqueous solutions of 13C-labeled C₁ and C₂ intermediates delivered to branches of the tropical species Inga edulis via the transpiration stream. Delivery of [13C]methanol and [13C]formaldehyde rapidly stimulated leaf emissions of [13C]methanol, [13C]formaldehyde, [13C]formic acid, and 13CO₂, confirming the existence of the C1 pathwaInternational journal of molecular sciencesIntegration of C₁ and C₂ Metabolism in Trees.PMC5666727DE-AC02-05CH11231Higuchi NChambers JQOikawa PNiinemets UFernandes de Souza VPorras RBill MJardine KJfalseIntegration of C₁ and C₂ Metabolism in Trees.C₁ metabolism in plants is known to be involved in photorespiration, nitrogen and amino acid metabolism, as well as methylation and biosynthesis of metabolites and biopolymers. Although the flux of carbon through the C₁ pathway is thought to be large, its intermediates are difficult to measure and relatively little is known about this potentially ubiquitous pathway. In this study, we evaluated the C₁ pathway and its integration with the central metabolism using aqueous solutions of 13C-labeled C₁ and C₂ intermediates delivered to branches of the tropical species Inga edulis via the transpiration stream. Delivery of [13C]methanol and [13C]formaldehyde rapidly stimulated leaf emissions of [13C]methanol, [13C]formaldehyde, [13C]formic acid, and 13CO₂, confirming the existence of the C1 pathwa2017-01-01T00:00:00Z2017 Sep2025-04-27T03:53:21.88Z2019-03-27T03:00:40ZS-EPMC56667272894662710.3390/ijms18102045