{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gabriel BM"],"funding":["Kuwait Ministry of Health","Saudi Ministry of Higher Education","Albert Renold Travel Fellowship","Novo Nordisk Foundation Challenge Grant","European Foundation for the Study of Diabetes","Wenner-Gren Foundation","NHS Grampian Endowment, Scotland, U.K.","European Social Fund under the Global Grant measure","Saltire scholarship"],"pagination":["e0185789"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5667803"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(11)"],"pubmed_abstract":["The H55N polymorphism in the Cs gene of A/J mice has been linked to low activity of the enzyme in skeletal muscles. The aim of the study was to test this hypothesis and examine effects of low citrate synthase (CS) activity on palmitate metabolism in muscle cells. Results of the study showed that carriers of the wild type (WT) Cs (C57BL/6J and Balb/cByJ mouse strains) had higher CS activity (p < 0.01) than carriers of the A/J variant (B6.A-(rs3676616-D10Utsw1)/KjnB6 and A/J mouse strains) in the heart, liver and gastrocnemius muscle. Furthermore, the recombinant CS protein of WT showed higher CS activity than the A/J variant. In C2C12 muscle cells the shRNA mediated 47% knockdown of CS activity reduced the rate of fatty acid oxidation compared to the control cells. In summary, our results a"],"journal":["PloS one"],"pubmed_title":["H55N polymorphism is associated with low citrate synthase activity which regulates lipid metabolism in mouse muscle cells."],"pmcid":["PMC5667803"],"funding_grant_id":["VP1-3.1-ŠMM-07-K-02-057","Ph.D. grant","EFSD New Horizons Collaborative Research Initiative","Postdoctoral Fellowship","2012/2013 Project No: 12/21"],"pubmed_authors":["Alhindi Y","Venckunas T","Al-Tarrah M","Ratkevicius A","Gabriel BM","Lionikas A","Kilikevicius A","Gray SR"],"additional_accession":[]},"is_claimable":false,"name":"H55N polymorphism is associated with low citrate synthase activity which regulates lipid metabolism in mouse muscle cells.","description":"The H55N polymorphism in the Cs gene of A/J mice has been linked to low activity of the enzyme in skeletal muscles. The aim of the study was to test this hypothesis and examine effects of low citrate synthase (CS) activity on palmitate metabolism in muscle cells. Results of the study showed that carriers of the wild type (WT) Cs (C57BL/6J and Balb/cByJ mouse strains) had higher CS activity (p < 0.01) than carriers of the A/J variant (B6.A-(rs3676616-D10Utsw1)/KjnB6 and A/J mouse strains) in the heart, liver and gastrocnemius muscle. Furthermore, the recombinant CS protein of WT showed higher CS activity than the A/J variant. In C2C12 muscle cells the shRNA mediated 47% knockdown of CS activity reduced the rate of fatty acid oxidation compared to the control cells. In summary, our results a","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-27T03:53:48.492Z","creation":"2019-03-27T03:00:43Z"},"accession":"S-EPMC5667803","cross_references":{"pubmed":["29095821"],"doi":["10.1371/journal.pone.0185789"]}}