{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zilberman Y"],"funding":["American Hospital Association","National Institutes of Health","NIGMS NIH HHS","NIH HHS"],"pagination":["3729-3744"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5674880"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["216(11)"],"pubmed_abstract":["During morphogenesis, adherens junctions (AJs) remodel to allow changes in cell shape and position while preserving adhesion. Here, we examine the function of Rho guanosine triphosphatase CDC-42 in AJ formation and regulation during <i>Caenorhabditis elegans</i> embryo elongation, a process driven by asymmetric epidermal cell shape changes. <i>cdc-42</i> mutant embryos arrest during elongation with epidermal ruptures. Unexpectedly, we find using time-lapse fluorescence imaging that <i>cdc-42</i> is not required for epidermal cell polarization or junction assembly, but rather is needed for proper junctional actin regulation during elongation. We show that the RhoGAP PAC-1/ARHGAP21 inhibits CDC-42 activity at AJs, and loss of PAC-1 or the interacting linker protein PICC-1/CCDC85A-C blocks el"],"journal":["The Journal of cell biology"],"pubmed_title":["Cdc42 regulates junctional actin but not cell polarization in the <i>Caenorhabditis elegans</i> epidermis."],"pmcid":["PMC5674880"],"funding_grant_id":["R01 GM078341","R35GM118081","R01GM078341","R35 GM118081","14POST18740059","P40 OD010440","R01GM098492","R01 GM098492"],"pubmed_authors":["Abrams J","Zilberman Y","Anderson DC","Nance J"],"additional_accession":[]},"is_claimable":false,"name":"Cdc42 regulates junctional actin but not cell polarization in the <i>Caenorhabditis elegans</i> epidermis.","description":"During morphogenesis, adherens junctions (AJs) remodel to allow changes in cell shape and position while preserving adhesion. Here, we examine the function of Rho guanosine triphosphatase CDC-42 in AJ formation and regulation during <i>Caenorhabditis elegans</i> embryo elongation, a process driven by asymmetric epidermal cell shape changes. <i>cdc-42</i> mutant embryos arrest during elongation with epidermal ruptures. Unexpectedly, we find using time-lapse fluorescence imaging that <i>cdc-42</i> is not required for epidermal cell polarization or junction assembly, but rather is needed for proper junctional actin regulation during elongation. We show that the RhoGAP PAC-1/ARHGAP21 inhibits CDC-42 activity at AJs, and loss of PAC-1 or the interacting linker protein PICC-1/CCDC85A-C blocks el","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-04-16T07:28:54.869Z","creation":"2019-10-30T08:12:52Z"},"accession":"S-EPMC5674880","cross_references":{"pubmed":["28903999"],"doi":["10.1083/jcb.201611061"]}}