biostudies-literatureKoseva BK-INBRENational Institutes of HealthKU GRFKU Botany EndowmentNIGMS NIH HHS1256-1267https://www.ebi.ac.uk/biostudies/studies/S-EPMC5675808biostudies-literatureUnknown216(4)Angiosperm diversity has been shaped by mating system evolution, with the most common transition from outcrossing to self-fertilizing. To investigate the genetic basis of this transition, we performed crosses between two species endemic to the Canary Islands, the self-compatible (SC) species Tolpis coronopifolia and its self-incompatible (SI) relative Tolpis santosii. We scored self-compatibility as self-seed set of recombinant plants within two F₂ populations. To map and genetically characterize the breakdown of SI, we built a draft genome sequence of T. coronopifolia, genotyped F₂ plants using multiplexed shotgun genotyping (MSG), and located MSG markers to the genome sequence. We identified a single quantitative trait locus (QTL) that explains nearly all variation The New phytologistThe genetic breakdown of sporophytic self-incompatibility in Tolpis coronopifolia (Asteraceae).PMC5675808P20 GM103418NIH P20-GM103418R01 GM073990-02R01 GM073990R01 GM073990‐02Kelly JKMort MECrawford DJBrown KEKoseva BfalseThe genetic breakdown of sporophytic self-incompatibility in Tolpis coronopifolia (Asteraceae).Angiosperm diversity has been shaped by mating system evolution, with the most common transition from outcrossing to self-fertilizing. To investigate the genetic basis of this transition, we performed crosses between two species endemic to the Canary Islands, the self-compatible (SC) species Tolpis coronopifolia and its self-incompatible (SI) relative Tolpis santosii. We scored self-compatibility as self-seed set of recombinant plants within two F₂ populations. To map and genetically characterize the breakdown of SI, we built a draft genome sequence of T. coronopifolia, genotyped F₂ plants using multiplexed shotgun genotyping (MSG), and located MSG markers to the genome sequence. We identified a single quantitative trait locus (QTL) that explains nearly all variation 2017-01-01T00:00:00Z2017 Dec2026-04-29T12:47:35.4Z2019-03-27T00:09:08ZS-EPMC56758082889215110.1111/nph.14759