biostudies-literatureUnknown8Esposito EPThe emergence of carbapenemase producing Enterobacteriaceae has raised major public health concern. The aim of this study was to investigate the molecular epidemiology and the mechanism of carbapenem resistance acquisition of multidrug-resistant <i>Klebsiella pneumoniae</i> isolates from 20 neonates in the neonatal intensive care unit (NICU) of the V. Monaldi Hospital in Naples, Italy, from April 2015 to March 2016. Genotype analysis by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) identified PFGE type A and subtypes A1 and A2 in 17, 2, and 1 isolates, respectively, and assigned all isolates to sequence type (ST) 104. <i>K. pneumoniae</i> isolates were resistant to all classes of β-lactams including carbapenems, fosfomycin, gentamicin, and trimethoprim-sulfFrontiers in microbiology2135https://www.ebi.ac.uk/biostudies/studies/S-EPMC5675864biostudies-literatureA Novel IncA/C1 Group Conjugative Plasmid, Encoding VIM-1 Metallo-Beta-Lactamase, Mediates the Acquisition of Carbapenem Resistance in ST104 <i>Klebsiella pneumoniae</i> Isolates from Neonates in the Intensive Care Unit of V. Monaldi Hospital in Naples.PMC5675864Cuccurullo SZarrilli RBernardo MMarone PDel Franco MEsposito EPTriassi MSassera DGaiarsa SPennino FCrivaro VfalseA Novel IncA/C1 Group Conjugative Plasmid, Encoding VIM-1 Metallo-Beta-Lactamase, Mediates the Acquisition of Carbapenem Resistance in ST104 <i>Klebsiella pneumoniae</i> Isolates from Neonates in the Intensive Care Unit of V. Monaldi Hospital in Naples.The emergence of carbapenemase producing Enterobacteriaceae has raised major public health concern. The aim of this study was to investigate the molecular epidemiology and the mechanism of carbapenem resistance acquisition of multidrug-resistant <i>Klebsiella pneumoniae</i> isolates from 20 neonates in the neonatal intensive care unit (NICU) of the V. Monaldi Hospital in Naples, Italy, from April 2015 to March 2016. Genotype analysis by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) identified PFGE type A and subtypes A1 and A2 in 17, 2, and 1 isolates, respectively, and assigned all isolates to sequence type (ST) 104. <i>K. pneumoniae</i> isolates were resistant to all classes of β-lactams including carbapenems, fosfomycin, gentamicin, and trimethoprim-sulf2017-01-01T00:00:00Z20172026-04-29T12:47:44.741Z2019-03-27T03:01:08ZS-EPMC56758642916342210.3389/fmicb.2017.02135