biostudies-literature00560Yeh HWNIBIB NIH HHSNIGMS NIH HHS971-974https://www.ebi.ac.uk/biostudies/studies/S-EPMC5678970biostudies-literatureUnknown14(10)Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.Nature methodsRed-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging.PMC5678970R21 EB021651R01 GM118675Ji AMartins-Green MMAi HWCarter DYeh HWKarmach O56falseRed-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging.Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.2017-01-01T00:00:00Z2017 Oct2024-11-15T08:50:53.499Z2019-03-26T23:06:44ZS-EPMC56789702886975610.1038/nmeth.4400