biostudies-literature00470Unknown8(1)Martineau MHoward Hughes Medical InstituteThe GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.Nature communications1412https://www.ebi.ac.uk/biostudies/studies/S-EPMC5680258biostudies-literatureSemisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.PMC5680258Grimm JBGruber TDLavis LDMartineau MSomasundaram ATaraska JWPerrais DChoquet D47falseSemisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.2017-01-01T00:00:00Z2017 Nov2024-11-21T01:07:17.049Z2019-03-27T03:01:27ZS-EPMC56802582912310210.1038/s41467-017-01752-5