{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Chaves-Pozo E"],"pubmed_abstract":["Nervous necrosis virus (NNV) causes high mortalities in several marine species. We aimed to evaluate the innate cell-mediated cytotoxic (CMC) activity of head-kidney leucocytes (HKLs) isolated from naïve European sea bass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), a very susceptible and resistant fish species to NNV, respectively, against fish cell lines infected with NNV. Seabream HKLs showed significantly increased innate CMC activity against NNV-infected cells, compared to those uninfected, while sea bass HKLs failed to do so. Thus, we performed a RNA-seq study to identify genes related to the CMC activity of sea bass leucocytes. Thus, we found that sea bass HKLs incubated with DLB-1 cells alone (CMC_DLB1) or with NNV-infected DLB-1 cells (CMC_DLB1-NNV) showed very si"],"journal":["Scientific reports"],"pagination":["15396"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5684396"],"repository":["biostudies-literature"],"pubmed_title":["Innate Cell-Mediated Cytotoxic Activity of European Sea Bass Leucocytes Against Nodavirus-Infected Cells: A Functional and RNA-seq Study."],"pmcid":["PMC5684396"],"pubmed_authors":["Cuesta A","Dabad M","Meseguer J","Valero Y","Esteve-Codina A","Gomez-Garrido J","Alioto T","Chaves-Pozo E","Esteban MA"],"additional_accession":[]},"is_claimable":false,"name":"Innate Cell-Mediated Cytotoxic Activity of European Sea Bass Leucocytes Against Nodavirus-Infected Cells: A Functional and RNA-seq Study.","description":"Nervous necrosis virus (NNV) causes high mortalities in several marine species. We aimed to evaluate the innate cell-mediated cytotoxic (CMC) activity of head-kidney leucocytes (HKLs) isolated from naïve European sea bass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), a very susceptible and resistant fish species to NNV, respectively, against fish cell lines infected with NNV. Seabream HKLs showed significantly increased innate CMC activity against NNV-infected cells, compared to those uninfected, while sea bass HKLs failed to do so. Thus, we performed a RNA-seq study to identify genes related to the CMC activity of sea bass leucocytes. Thus, we found that sea bass HKLs incubated with DLB-1 cells alone (CMC_DLB1) or with NNV-infected DLB-1 cells (CMC_DLB1-NNV) showed very si","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-04-16T09:30:57.969Z","creation":"2019-03-27T03:01:48Z"},"accession":"S-EPMC5684396","cross_references":{"pubmed":["29133947"],"doi":["10.1038/s41598-017-15629-6"]}}