biostudies-literatureUnknown8(1)Chen ACIHRCytokinesis is initiated by the localized assembly of the contractile ring, a dynamic actomyosin structure that generates a membrane furrow between the segregating chromosomal masses to divide a cell into two. Here we show that the stabilization and organization of the cytokinetic furrow is specifically dependent on localized β-actin filament assembly at the site of cytokinesis. β-actin filaments are assembled directly at the furrow by an anillin-dependent pathway that enhances RhoA-dependent activation of the formin DIAPH3, an actin nucleator. DIAPH3 specifically generates homopolymeric filaments of β-actin in vitro. By employing enhancers and activators, cells can achieve acute spatio-temporal control over isoform-specific actin arrays that are required for distinct cellular functions.Nature communications1530https://www.ebi.ac.uk/biostudies/studies/S-EPMC5691081biostudies-literatureCytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.PMC5691081Chen AWilde AArora PDMcCulloch CAfalseCytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.Cytokinesis is initiated by the localized assembly of the contractile ring, a dynamic actomyosin structure that generates a membrane furrow between the segregating chromosomal masses to divide a cell into two. Here we show that the stabilization and organization of the cytokinetic furrow is specifically dependent on localized β-actin filament assembly at the site of cytokinesis. β-actin filaments are assembled directly at the furrow by an anillin-dependent pathway that enhances RhoA-dependent activation of the formin DIAPH3, an actin nucleator. DIAPH3 specifically generates homopolymeric filaments of β-actin in vitro. By employing enhancers and activators, cells can achieve acute spatio-temporal control over isoform-specific actin arrays that are required for distinct cellular functions.2017-01-01T00:00:00Z2017 Nov2025-04-04T14:03:26.7Z2019-03-27T03:02:16ZS-EPMC56910812914691110.1038/s41467-017-01231-x