{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dumas L"],"funding":["Agence Nationale de la Recherche","Institut National des Sciences de l&apos;Ingénierie et des Systèmes","Initiative d&apos;Excellence"],"pagination":["12063-12068"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5692589"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["114(45)"],"pubmed_abstract":["The cytochrome (cyt) <i>b</i><sub>6</sub><i>f</i> complex and Stt7 kinase regulate the antenna sizes of photosystems I and II through state transitions, which are mediated by a reversible phosphorylation of light harvesting complexes II, depending on the redox state of the plastoquinone pool. When the pool is reduced, the cyt <i>b</i><sub>6</sub><i>f</i> activates the Stt7 kinase through a mechanism that is still poorly understood. After random mutagenesis of the chloroplast <i>petD</i> gene, coding for subunit IV of the cyt <i>b</i><sub>6</sub><i>f</i> complex, and complementation of a Δ<i>petD</i> host strain by chloroplast transformation, we screened for impaired state transitions in vivo by chlorophyll fluorescence imaging. We show that residues Asn122, Tyr124, and Arg125 in the stroma"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["A stromal region of cytochrome <i>b</i><sub>6</sub><i>f</i> subunit IV is involved in the activation of the Stt7 kinase in <i>Chlamydomonas</i>."],"pmcid":["PMC5692589"],"funding_grant_id":["ANR-11-LABEX-0011-01","ANR-14-CE05-0041-01","PhotoModes 72749"],"pubmed_authors":["Zito F","Peltier G","Alric J","Dumas L","Auroy P","Blangy S","Johnson X"],"additional_accession":[]},"is_claimable":false,"name":"A stromal region of cytochrome <i>b</i><sub>6</sub><i>f</i> subunit IV is involved in the activation of the Stt7 kinase in <i>Chlamydomonas</i>.","description":"The cytochrome (cyt) <i>b</i><sub>6</sub><i>f</i> complex and Stt7 kinase regulate the antenna sizes of photosystems I and II through state transitions, which are mediated by a reversible phosphorylation of light harvesting complexes II, depending on the redox state of the plastoquinone pool. When the pool is reduced, the cyt <i>b</i><sub>6</sub><i>f</i> activates the Stt7 kinase through a mechanism that is still poorly understood. After random mutagenesis of the chloroplast <i>petD</i> gene, coding for subunit IV of the cyt <i>b</i><sub>6</sub><i>f</i> complex, and complementation of a Δ<i>petD</i> host strain by chloroplast transformation, we screened for impaired state transitions in vivo by chlorophyll fluorescence imaging. We show that residues Asn122, Tyr124, and Arg125 in the stroma","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-05-02T02:29:03.42Z","creation":"2019-03-26T23:35:56Z"},"accession":"S-EPMC5692589","cross_references":{"pubmed":["29078388"],"doi":["10.1073/pnas.1713343114"]}}