{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matheson LS"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["1550"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5698286"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8"],"pubmed_abstract":["V(D)J recombination is essential for the generation of diverse antigen receptor (AgR) repertoires. In B cells, immunoglobulin kappa (<i>Igκ</i>) light chain recombination follows immunoglobulin heavy chain (<i>Igh</i>) recombination. We recently developed the DNA-based VDJ-seq assay for the unbiased quantitation of <i>Igh</i> VH and DH repertoires. Integration of VDJ-seq data with genome-wide datasets revealed that two chromatin states at the recombination signal sequence (RSS) of VH genes are highly predictive of recombination in mouse pro-B cells. It is unknown whether local chromatin states contribute to Vκ gene choice during <i>Igκ</i> recombination. Here we adapt VDJ-seq to profile the <i>Igκ</i> VκJκ repertoire and present a comprehensive readout in mouse pre-B cells, revealing highl"],"journal":["Frontiers in immunology"],"pubmed_title":["Local Chromatin Features Including PU.1 and IKAROS Binding and H3K4 Methylation Shape the Repertoire of Immunoglobulin Kappa Genes Chosen for V(D)J Recombination."],"pmcid":["PMC5698286"],"funding_grant_id":["BB/K010867/1","BBS/E/B/000C0428","1520397","BBS/E/B/000C0405","BBS/E/B/000C0427","BBS/E/B/000C0404"],"pubmed_authors":["Bolland DJ","Corcoran AE","Krueger F","Matheson LS","Andrews S","Chovanec P","Koohy H"],"additional_accession":[]},"is_claimable":false,"name":"Local Chromatin Features Including PU.1 and IKAROS Binding and H3K4 Methylation Shape the Repertoire of Immunoglobulin Kappa Genes Chosen for V(D)J Recombination.","description":"V(D)J recombination is essential for the generation of diverse antigen receptor (AgR) repertoires. In B cells, immunoglobulin kappa (<i>Igκ</i>) light chain recombination follows immunoglobulin heavy chain (<i>Igh</i>) recombination. We recently developed the DNA-based VDJ-seq assay for the unbiased quantitation of <i>Igh</i> VH and DH repertoires. Integration of VDJ-seq data with genome-wide datasets revealed that two chromatin states at the recombination signal sequence (RSS) of VH genes are highly predictive of recombination in mouse pro-B cells. It is unknown whether local chromatin states contribute to Vκ gene choice during <i>Igκ</i> recombination. Here we adapt VDJ-seq to profile the <i>Igκ</i> VκJκ repertoire and present a comprehensive readout in mouse pre-B cells, revealing highl","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-04-07T15:58:15.977Z","creation":"2019-03-27T03:02:39Z"},"accession":"S-EPMC5698286","cross_references":{"pubmed":["29204143"],"doi":["10.3389/fimmu.2017.01550"]}}