biostudies-literatureMatheson LSBiotechnology and Biological Sciences Research Council1550https://www.ebi.ac.uk/biostudies/studies/S-EPMC5698286biostudies-literatureUnknown8V(D)J recombination is essential for the generation of diverse antigen receptor (AgR) repertoires. In B cells, immunoglobulin kappa (<i>Igκ</i>) light chain recombination follows immunoglobulin heavy chain (<i>Igh</i>) recombination. We recently developed the DNA-based VDJ-seq assay for the unbiased quantitation of <i>Igh</i> VH and DH repertoires. Integration of VDJ-seq data with genome-wide datasets revealed that two chromatin states at the recombination signal sequence (RSS) of VH genes are highly predictive of recombination in mouse pro-B cells. It is unknown whether local chromatin states contribute to Vκ gene choice during <i>Igκ</i> recombination. Here we adapt VDJ-seq to profile the <i>Igκ</i> VκJκ repertoire and present a comprehensive readout in mouse pre-B cells, revealing highlFrontiers in immunologyLocal Chromatin Features Including PU.1 and IKAROS Binding and H3K4 Methylation Shape the Repertoire of Immunoglobulin Kappa Genes Chosen for V(D)J Recombination.PMC5698286BB/K010867/1BBS/E/B/000C04281520397BBS/E/B/000C0405BBS/E/B/000C0427BBS/E/B/000C0404Bolland DJCorcoran AEKrueger FMatheson LSAndrews SChovanec PKoohy HfalseLocal Chromatin Features Including PU.1 and IKAROS Binding and H3K4 Methylation Shape the Repertoire of Immunoglobulin Kappa Genes Chosen for V(D)J Recombination.V(D)J recombination is essential for the generation of diverse antigen receptor (AgR) repertoires. In B cells, immunoglobulin kappa (<i>Igκ</i>) light chain recombination follows immunoglobulin heavy chain (<i>Igh</i>) recombination. We recently developed the DNA-based VDJ-seq assay for the unbiased quantitation of <i>Igh</i> VH and DH repertoires. Integration of VDJ-seq data with genome-wide datasets revealed that two chromatin states at the recombination signal sequence (RSS) of VH genes are highly predictive of recombination in mouse pro-B cells. It is unknown whether local chromatin states contribute to Vκ gene choice during <i>Igκ</i> recombination. Here we adapt VDJ-seq to profile the <i>Igκ</i> VκJκ repertoire and present a comprehensive readout in mouse pre-B cells, revealing highl2017-01-01T00:00:00Z20172026-04-07T15:58:15.977Z2019-03-27T03:02:39ZS-EPMC56982862920414310.3389/fimmu.2017.01550