{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cattoni DI"],"funding":["European Research Council"],"pagination":["1753"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5700980"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["At the kilo- to megabase pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate to form nuclear compartments. Here, we combine high-content super-resolution microscopies with state-of-the-art DNA-labeling methods to reveal the variability in the multiscale organization of the Drosophila genome. We find that association frequencies within TADs and between TAD borders are below ~10%, independently of TAD size, epigenetic state, or cell type. Critically, despite this large heterogeneity, we are able to visualize nanometer-sized epigenetic domains at the single-cell level. In addition, absolute contact frequencies within and between TADs are to a large extent defined by genomic distance, higher-order chromosome ar"],"journal":["Nature communications"],"pubmed_title":["Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions."],"pmcid":["PMC5700980"],"funding_grant_id":["260787","609989"],"pubmed_authors":["Marti-Renom MA","Cavalli G","Di Stefano M","Fiche JB","Valeri A","Houbron C","Chamousset D","Sexton T","Cattoni DI","Georgieva M","Chang JM","Cardozo Gizzi AM","Nollmann M","Gonzalez I","Dejardin S","Bantignies F"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions.","description":"At the kilo- to megabase pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate to form nuclear compartments. Here, we combine high-content super-resolution microscopies with state-of-the-art DNA-labeling methods to reveal the variability in the multiscale organization of the Drosophila genome. We find that association frequencies within TADs and between TAD borders are below ~10%, independently of TAD size, epigenetic state, or cell type. Critically, despite this large heterogeneity, we are able to visualize nanometer-sized epigenetic domains at the single-cell level. In addition, absolute contact frequencies within and between TADs are to a large extent defined by genomic distance, higher-order chromosome ar","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-04-16T07:18:34.433Z","creation":"2019-03-27T03:02:50Z"},"accession":"S-EPMC5700980","cross_references":{"pubmed":["29170434"],"doi":["10.1038/s41467-017-01962-x"]}}