{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["292(47)"],"submitter":["Pierman B"],"pubmed_abstract":["Within the plant ATP-binding cassette transporter family, pleiotropic drug resistance (PDR) transporters play essential functions, such as in hormone transport or defense against biotic and abiotic stresses. NtPDR1 from <i>Nicotiana tabacum</i> has been shown to be involved in the constitutive defense against pathogens through the secretion of toxic cyclic diterpenes, such as the antimicrobial substrates cembrene and sclareol from the leaf hairs (trichomes). However, direct evidence of an interaction between NtPDR1 and terpenes is lacking. Here, we stably expressed NtPDR1 in <i>N. tabacum</i> BY-2 suspension cells. NtPDR1 was purified as an active monomer glycosylated at a single site in the third external loop. NtPDR1 reconstitution in proteoliposomes stimulated its basal ATPase activity "],"journal":["The Journal of biological chemistry"],"pagination":["19491-19502"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5702685"],"repository":["biostudies-literature"],"pubmed_title":["Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses."],"pmcid":["PMC5702685"],"pubmed_authors":["Toussaint F","Boutry M","Bertin A","Smargiasso N","Levy D","De Pauw E","Pierman B"],"additional_accession":[]},"is_claimable":false,"name":"Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses.","description":"Within the plant ATP-binding cassette transporter family, pleiotropic drug resistance (PDR) transporters play essential functions, such as in hormone transport or defense against biotic and abiotic stresses. NtPDR1 from <i>Nicotiana tabacum</i> has been shown to be involved in the constitutive defense against pathogens through the secretion of toxic cyclic diterpenes, such as the antimicrobial substrates cembrene and sclareol from the leaf hairs (trichomes). However, direct evidence of an interaction between NtPDR1 and terpenes is lacking. Here, we stably expressed NtPDR1 in <i>N. tabacum</i> BY-2 suspension cells. NtPDR1 was purified as an active monomer glycosylated at a single site in the third external loop. NtPDR1 reconstitution in proteoliposomes stimulated its basal ATPase activity ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2026-04-16T17:27:10.304Z","creation":"2019-03-27T00:08:34Z"},"accession":"S-EPMC5702685","cross_references":{"pubmed":["28972149"],"doi":["10.1074/jbc.m117.811935","10.1074/jbc.M117.811935"]}}