biostudies-literatureKlein RHNEI NIH HHSU.S. National Library of MedicineNational Cancer InstituteNCI NIH HHSNational Institutes of HealthNIAMS NIH HHS18937-18950https://www.ebi.ac.uk/biostudies/studies/S-EPMC5704477biostudies-literatureUnknown292(46)During tissue development, transcription factors bind regulatory DNA regions called enhancers, often located at great distances from the genes they regulate, to control gene expression. The enhancer landscape during embryonic stem cell differentiation has been well characterized. By contrast, little is known about the shared and unique enhancer regulatory mechanisms in different ectodermally derived epithelial cells. Here we use ChIP sequencing (ChIP-seq) to identify domains enriched for the histone marks histone H3 lysine 4 trimethylation, histone H3 lysine 4 monomethylation, and histone H3 lysine 27 acetylation (H3K4me3, H3K4me1, and H3K27ac) and define, for the first time, the super enhancers and typical enhancers active in primary human corneal epithelial cells. We show that regulatoryThe Journal of biological chemistryCharacterization of enhancers and the role of the transcription factor KLF7 in regulating corneal epithelial differentiation.PMC57044775T32CA09054–38R01AR44882R01EY019413T15LM00744R01 EY019413T32 CA009054R01 AR044882Klein RHKashgari GHu WDoan MNguyen TLin ZAndersen BfalseCharacterization of enhancers and the role of the transcription factor KLF7 in regulating corneal epithelial differentiation.During tissue development, transcription factors bind regulatory DNA regions called enhancers, often located at great distances from the genes they regulate, to control gene expression. The enhancer landscape during embryonic stem cell differentiation has been well characterized. By contrast, little is known about the shared and unique enhancer regulatory mechanisms in different ectodermally derived epithelial cells. Here we use ChIP sequencing (ChIP-seq) to identify domains enriched for the histone marks histone H3 lysine 4 trimethylation, histone H3 lysine 4 monomethylation, and histone H3 lysine 27 acetylation (H3K4me3, H3K4me1, and H3K27ac) and define, for the first time, the super enhancers and typical enhancers active in primary human corneal epithelial cells. We show that regulatory2017-01-01T00:00:00Z2017 Nov2026-05-04T05:06:48.908Z2019-03-27T00:07:25ZS-EPMC57044772891672510.1074/jbc.M117.79311710.1074/jbc.m117.793117