{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kawaguchi M"],"funding":["Ministry of Education, Science, Sports and Culture, Japan","NCI NIH HHS"],"pagination":["1610-23"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5707200"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["185(6)"],"pubmed_abstract":["Hepatocyte growth factor activator inhibitor type 1 (HAI-1; official symbol SPINT1) is a membrane-associated serine proteinase inhibitor abundantly expressed in epithelial tissues. Genetically engineered mouse models demonstrated that HAI-1 is critical for epidermal function, possibly through direct and indirect regulation of cell surface proteases, such as matriptase and prostasin. To obtain a better understanding of the role of HAI-1 in maintaining epidermal integrity, we performed ultrastructural analysis of Spint1-deleted mouse epidermis and organotypic culture of an HAI-1 knockdown (KD) human keratinocyte cell line, HaCaT. We found that the aggregation of tonofilaments to desmosomes was significantly reduced in HAI-1-deficient mouse epidermis with decreased desmosome number. Similar f"],"journal":["The American journal of pathology"],"pubmed_title":["Hepatocyte growth factor activator inhibitor type 1 maintains the assembly of keratin into desmosomes in keratinocytes by regulating protease-activated receptor 2-dependent p38 signaling."],"pmcid":["PMC5707200"],"funding_grant_id":["25860166","R01 CA123223","24390099","P30 CA051008"],"pubmed_authors":["Kanemaru A","Kawaguchi M","Yamamoto K","Baba T","Sawaguchi A","Johnson MD","Lin CY","Fukushima T","Kataoka H"],"additional_accession":[]},"is_claimable":false,"name":"Hepatocyte growth factor activator inhibitor type 1 maintains the assembly of keratin into desmosomes in keratinocytes by regulating protease-activated receptor 2-dependent p38 signaling.","description":"Hepatocyte growth factor activator inhibitor type 1 (HAI-1; official symbol SPINT1) is a membrane-associated serine proteinase inhibitor abundantly expressed in epithelial tissues. Genetically engineered mouse models demonstrated that HAI-1 is critical for epidermal function, possibly through direct and indirect regulation of cell surface proteases, such as matriptase and prostasin. To obtain a better understanding of the role of HAI-1 in maintaining epidermal integrity, we performed ultrastructural analysis of Spint1-deleted mouse epidermis and organotypic culture of an HAI-1 knockdown (KD) human keratinocyte cell line, HaCaT. We found that the aggregation of tonofilaments to desmosomes was significantly reduced in HAI-1-deficient mouse epidermis with decreased desmosome number. Similar f","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jun","modification":"2025-04-04T13:36:01.254Z","creation":"2019-03-27T03:03:20Z"},"accession":"S-EPMC5707200","cross_references":{"pubmed":["25842366"],"doi":["10.1016/j.ajpath.2015.02.009"]}}