{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Berry MH"],"funding":["NEI NIH HHS","European Research Council","NCRR NIH HHS","NINDS NIH HHS"],"pagination":["1862"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5709376"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Retinitis pigmentosa results in blindness due to degeneration of photoreceptors, but spares other retinal cells, leading to the hope that expression of light-activated signaling proteins in the surviving cells could restore vision. We used a retinal G protein-coupled receptor, mGluR2, which we chemically engineered to respond to light. In retinal ganglion cells (RGCs) of blind rd1 mice, photoswitch-charged mGluR2 (\"SNAG-mGluR2\") evoked robust OFF responses to light, but not in wild-type retinas, revealing selectivity for RGCs that have lost photoreceptor input. SNAG-mGluR2 enabled animals to discriminate parallel from perpendicular lines and parallel lines at varying spacing. Simultaneous viral delivery of the inhibitory SNAG-mGluR2 and excitatory light-activated ionotropic glutamate receptor LiGluR yielded a distribution of expression ratios, restoration of ON, OFF and ON-OFF light responses and improved visual acuity. Thus, SNAG-mGluR2 restores patterned vision and combinatorial light response diversity provides a new logic for enhanced-acuity retinal prosthetics."],"journal":["Nature communications"],"pubmed_title":["Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor."],"pmcid":["PMC5709376"],"funding_grant_id":["T32 NS095939","PN2 EY018241","268795","S10 RR028971"],"pubmed_authors":["Cao K","Kramer RH","Berry MH","Isacoff EY","Levitz J","Broichhagen J","Aghi K","Trauner D","Flannery J","Kim YJ","Gaub BM","Holt A","Visel M","Stanley C"],"additional_accession":[]},"is_claimable":false,"name":"Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor.","description":"Retinitis pigmentosa results in blindness due to degeneration of photoreceptors, but spares other retinal cells, leading to the hope that expression of light-activated signaling proteins in the surviving cells could restore vision. We used a retinal G protein-coupled receptor, mGluR2, which we chemically engineered to respond to light. In retinal ganglion cells (RGCs) of blind rd1 mice, photoswitch-charged mGluR2 (\"SNAG-mGluR2\") evoked robust OFF responses to light, but not in wild-type retinas, revealing selectivity for RGCs that have lost photoreceptor input. SNAG-mGluR2 enabled animals to discriminate parallel from perpendicular lines and parallel lines at varying spacing. Simultaneous viral delivery of the inhibitory SNAG-mGluR2 and excitatory light-activated ionotropic glutamate receptor LiGluR yielded a distribution of expression ratios, restoration of ON, OFF and ON-OFF light responses and improved visual acuity. Thus, SNAG-mGluR2 restores patterned vision and combinatorial light response diversity provides a new logic for enhanced-acuity retinal prosthetics.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Nov","modification":"2024-11-20T14:17:41.143Z","creation":"2019-03-26T23:12:22Z"},"accession":"S-EPMC5709376","cross_references":{"pubmed":["29192252"],"doi":["10.1038/s41467-017-01990-7"]}}