biostudies-literatureUnknown17(1)Nguyen MTVietnam National University (VNU-HCM)<h4>Background</h4>The holistic approach of traditional medicine renders the identification of its mechanisms of action difficult. Microarray technology provides an efficient way to analyze the complex genome-wide gene expression of cells treated with mixtures of medicinal ingredients. We performed transcriptional profiling of MCF-7 cells treated with Nam Dia Long (NDL), a Vietnamese traditional formula, to explore the mechanism of action underlying the apoptosis inducing effect of this formula reported in a previous study.<h4>Methods</h4>MCF-7 cells were treated with aqueous extracts of NDL at the IC₅₀ concentration for 24, 36 and 48 h. Total RNAs at 24 h and 48 h were subsequently extracted, reverse transcribed and submitted to microarray expression profiling using the Human HBMC complementary and alternative medicine522https://www.ebi.ac.uk/biostudies/studies/S-EPMC5716261biostudies-literatureNam Dia long, a Vietnamese folk formula, induces apoptosis in MCF-7 cells through various mechanisms of action.PMC5716261Ho-Huynh TDNguyen MTfalseNam Dia long, a Vietnamese folk formula, induces apoptosis in MCF-7 cells through various mechanisms of action.<h4>Background</h4>The holistic approach of traditional medicine renders the identification of its mechanisms of action difficult. Microarray technology provides an efficient way to analyze the complex genome-wide gene expression of cells treated with mixtures of medicinal ingredients. We performed transcriptional profiling of MCF-7 cells treated with Nam Dia Long (NDL), a Vietnamese traditional formula, to explore the mechanism of action underlying the apoptosis inducing effect of this formula reported in a previous study.<h4>Methods</h4>MCF-7 cells were treated with aqueous extracts of NDL at the IC₅₀ concentration for 24, 36 and 48 h. Total RNAs at 24 h and 48 h were subsequently extracted, reverse transcribed and submitted to microarray expression profiling using the Human H2017-01-01T00:00:00Z2017 Dec2025-04-18T18:05:53.704Z2019-03-27T03:03:55ZS-EPMC57162612920277510.1186/s12906-017-2027-2