{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wollison BM"],"funding":["Office of Extramural Research, National Institutes of Health (US)","Team Fernando&apos;s Fight","Go 4 the Goal Foundation","NCI NIH HHS","Children&apos;s Oncology GroupTranslational Pilot Studies Program for Solid Malignancies","Nate Cavallo Fund"],"pagination":["e0188835"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5716571"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(12)"],"pubmed_abstract":["<h4>Objectives</h4>Liquid biopsy technologies allow non-invasive tumor profiling for patients with solid tumor malignancies by sequencing circulating tumor DNA. These studies may be useful in risk-stratification, monitoring for relapse, and understanding tumor evolution. The quality of DNA obtained for these studies is improved when blood samples are collected in tubes that stabilizing white blood cells (WBC). However, ongoing germline research in pediatric oncology generally requires obtaining blood samples in EDTA tubes, which do not contain a WBC-stabilizing preservative. In this study, we explored whether blood samples collected in WBC-stabilizing tubes could be used for both liquid biopsy and germline studies simultaneously, minimizing blood collection volumes for pediatric patients.<"],"journal":["PloS one"],"pubmed_title":["Blood collection in cell-stabilizing tubes does not impact germline DNA quality for pediatric patients."],"pmcid":["PMC5716571"],"funding_grant_id":["K08 CA188073","5K08CA188073-03"],"pubmed_authors":["Clapp A","Wollison BM","Crompton BD","Mckinney A","Clinton C","Thorner AR","Gastier-Foster JM","Ward A","Nag A","Thai E"],"additional_accession":[]},"is_claimable":false,"name":"Blood collection in cell-stabilizing tubes does not impact germline DNA quality for pediatric patients.","description":"<h4>Objectives</h4>Liquid biopsy technologies allow non-invasive tumor profiling for patients with solid tumor malignancies by sequencing circulating tumor DNA. These studies may be useful in risk-stratification, monitoring for relapse, and understanding tumor evolution. The quality of DNA obtained for these studies is improved when blood samples are collected in tubes that stabilizing white blood cells (WBC). However, ongoing germline research in pediatric oncology generally requires obtaining blood samples in EDTA tubes, which do not contain a WBC-stabilizing preservative. In this study, we explored whether blood samples collected in WBC-stabilizing tubes could be used for both liquid biopsy and germline studies simultaneously, minimizing blood collection volumes for pediatric patients.<","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-27T03:05:44.155Z","creation":"2019-03-27T03:03:56Z"},"accession":"S-EPMC5716571","cross_references":{"pubmed":["29206863"],"doi":["10.1371/journal.pone.0188835"]}}