biostudies-literatureChiasson DMDeutsche ForschungsgemeinschaftEuropean Research CouncilAlexander von Humboldt-Stiftunge25012https://www.ebi.ac.uk/biostudies/studies/S-EPMC5716663biostudies-literatureUnknown6The coordinated control of Ca²⁺ signaling is essential for development in eukaryotes. Cyclic nucleotide-gated channel (CNGC) family members mediate Ca²⁺ influx from cellular stores in plants (Charpentier et al., 2016; Gao et al., 2016; Frietsch et al., 2007; Urquhart et al., 2007). Here, we report the unusual genetic behavior of a quantitative gain-of-function <i>CNGC</i> mutation (<i>brush</i>) in <i>Lotus japonicus</i> resulting in a leaky tetrameric channel. <i>brush</i> resides in a cluster of redundant <i>CNGCs</i> encoding subunits which resemble metazoan voltage-gated potassium (Kv1-Kv4) channels in assembly and gating properties. The recessive mongenic <i>brush</i> mutation impaired root development and infection by nitrogen-fixing rhizobia. The <i>brush</i> aeLifeA quantitative hypermorphic <i>CNGC</i> allele confers ectopic calcium flux and impairs cellular development.PMC5716663340904 (EvolvingNodules)FOR964 (Calcium)340904Chiasson DMBrachmann AParniske MSollweck KDietrich PHaage KfalseA quantitative hypermorphic <i>CNGC</i> allele confers ectopic calcium flux and impairs cellular development.The coordinated control of Ca²⁺ signaling is essential for development in eukaryotes. Cyclic nucleotide-gated channel (CNGC) family members mediate Ca²⁺ influx from cellular stores in plants (Charpentier et al., 2016; Gao et al., 2016; Frietsch et al., 2007; Urquhart et al., 2007). Here, we report the unusual genetic behavior of a quantitative gain-of-function <i>CNGC</i> mutation (<i>brush</i>) in <i>Lotus japonicus</i> resulting in a leaky tetrameric channel. <i>brush</i> resides in a cluster of redundant <i>CNGCs</i> encoding subunits which resemble metazoan voltage-gated potassium (Kv1-Kv4) channels in assembly and gating properties. The recessive mongenic <i>brush</i> mutation impaired root development and infection by nitrogen-fixing rhizobia. The <i>brush</i> a2017-01-01T00:00:00Z2017 Sep2026-05-05T21:11:56.979Z2019-03-27T03:03:57ZS-EPMC57166632893369210.7554/eLife.25012