biostudies-literatureUnknown8Titus JKSnakebite envenomation is an important global health concern. The current standard treatment approach for snakebite envenomation relies on antibody-based antisera, which are expensive, not universally available, and can lead to adverse physiological effects. Phage display techniques offer a powerful tool for the selection of phage-expressed peptides, which can bind with high specificity and affinity towards venom components. In this research, the amino acid sequences of Phospholipase A2 (PLA2) from multiple cottonmouth species were analyzed, and a consensus peptide synthesized. Three phage display libraries were panned against this consensus peptide, crosslinked to capillary tubes, followed by a modified surface panning procedure. This high throughput selection method identified four phageJournal of venom research19-24https://www.ebi.ac.uk/biostudies/studies/S-EPMC5735792biostudies-literatureApplication of phage display for the development of a novel inhibitor of PLA2 activity in Western cottonmouth venom.PMC5735792Kay MKTitus JKGlaser CJJfalseApplication of phage display for the development of a novel inhibitor of PLA2 activity in Western cottonmouth venom.Snakebite envenomation is an important global health concern. The current standard treatment approach for snakebite envenomation relies on antibody-based antisera, which are expensive, not universally available, and can lead to adverse physiological effects. Phage display techniques offer a powerful tool for the selection of phage-expressed peptides, which can bind with high specificity and affinity towards venom components. In this research, the amino acid sequences of Phospholipase A2 (PLA2) from multiple cottonmouth species were analyzed, and a consensus peptide synthesized. Three phage display libraries were panned against this consensus peptide, crosslinked to capillary tubes, followed by a modified surface panning procedure. This high throughput selection method identified four phage2017-01-01T00:00:00Z20172025-04-27T02:58:49.393Z2019-03-26T22:50:00ZS-EPMC573579229285351