{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vanhara P"],"funding":["National Program of Sustainability II","Agency of the Czech Republic","Agency of Masaryk University"],"pagination":["109-114"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5746154"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. T"],"journal":["Stem cells translational medicine"],"pubmed_title":["Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells."],"pmcid":["PMC5746154"],"funding_grant_id":["LQ1605","MEYS CR","GA15-11707S","MUNI/A/1369/2016"],"pubmed_authors":["Kucera L","Prokes L","Hampl A","Vanhara P","Pena-Mendez EM","Havel J","Jureckova L"],"additional_accession":[]},"is_claimable":false,"name":"Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells.","description":"The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. T","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jan","modification":"2026-04-08T01:06:51.13Z","creation":"2019-03-27T03:05:56Z"},"accession":"S-EPMC5746154","cross_references":{"pubmed":["29248004"],"doi":["10.1002/sctm.17-0107"]}}