{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["Ong L"],"funding":["Univeristy of Otago"],"pubmed_abstract":["Mutations in the cytochrome c gene (CYCS) cause autosomal dominant thrombocytopenia by an unknown mechanism. While attempting to generate megakaryoblastic cell lines exogenously expressing cytochrome c variants, we discovered that endogenous cytochrome c expression increased both upon induction of differentiation with the phorbol ester phorbol 12-myristate 13-acetate (PMA), and as cell density increased. A concomitant increase in cytochrome c oxidase subunit II in response to PMA, but not cell higher cell density, suggests upregulation of the mitochondrial respiratory chain may be a specific feature of differentiation. These results highlight the likely importance of cytochrome c in both differentiating and proliferating cells, and illustrate the unsuitability of megakaryoblastic lines for"],"journal":["PloS one"],"pagination":["e0190433"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5747465"],"repository":["biostudies-literature"],"pubmed_title":["Differentiation and cell density upregulate cytochrome c levels in megakaryoblastic cell lines: Implications for analysis of CYCS-associated thrombocytopenia."],"pmcid":["PMC5747465"],"pubmed_authors":["Ledgerwood EC","Ong L","McDonald KO"],"additional_accession":[]},"is_claimable":false,"name":"Differentiation and cell density upregulate cytochrome c levels in megakaryoblastic cell lines: Implications for analysis of CYCS-associated thrombocytopenia.","description":"Mutations in the cytochrome c gene (CYCS) cause autosomal dominant thrombocytopenia by an unknown mechanism. While attempting to generate megakaryoblastic cell lines exogenously expressing cytochrome c variants, we discovered that endogenous cytochrome c expression increased both upon induction of differentiation with the phorbol ester phorbol 12-myristate 13-acetate (PMA), and as cell density increased. A concomitant increase in cytochrome c oxidase subunit II in response to PMA, but not cell higher cell density, suggests upregulation of the mitochondrial respiratory chain may be a specific feature of differentiation. These results highlight the likely importance of cytochrome c in both differentiating and proliferating cells, and illustrate the unsuitability of megakaryoblastic lines for","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-05-05T21:15:21.842Z","creation":"2019-03-26T22:58:46Z"},"accession":"S-EPMC5747465","cross_references":{"pubmed":["29287084"],"doi":["10.1371/journal.pone.0190433"]}}