{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Resendez A"],"funding":["U.S. Department of Health and Human Services","NIGMS NIH HHS","National Science Foundation"],"pagination":["5444-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5747966"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(10)"],"pubmed_abstract":["With the aim of discerning between different sugar and sugar alcohols of biomedical relevance, such as gut permeability, arrays of 2-component probes were assembled with up to six boronic acid-appended viologens (BBVs): 4,4'-o-BBV, 3,3'-o-BBV, 3,4'-o-BBV, 4,4'-o,m-BBV, 4,7'-o-PBBV, and pBoB, each coupled to the fluorophore 8-hydroxypyrene, 1,3,6-trisulfonic acid trisodium salt (HPTS). These probes were screened for their ability to discriminate between lactulose, l-rhamnose, 3-O-methyl-d-glucose, and xylose. Binding studies of sugar alcohols mannitol, sorbitol, erythritol, adonitol, arabitol, galactitol, and xylitol revealed that diols containing threo-1,2-diol units have higher affinity for BBVs relative diols containing erythro-1,2 units. Those containing both threo-1,2- and 1,3-syn diol"],"journal":["Analytical chemistry"],"pubmed_title":["Multiwell Assay for the Analysis of Sugar Gut Permeability Markers: Discrimination of Sugar Alcohols with a Fluorescent Probe Array Based on Boronic Acid Appended Viologens."],"pmcid":["PMC5747966"],"funding_grant_id":["5R25GM058903","R25 GM058903"],"pubmed_authors":["Singaram B","Joseph J","Panescu P","Webb DL","Resendez A","Banda I","Zuniga R"],"additional_accession":[]},"is_claimable":false,"name":"Multiwell Assay for the Analysis of Sugar Gut Permeability Markers: Discrimination of Sugar Alcohols with a Fluorescent Probe Array Based on Boronic Acid Appended Viologens.","description":"With the aim of discerning between different sugar and sugar alcohols of biomedical relevance, such as gut permeability, arrays of 2-component probes were assembled with up to six boronic acid-appended viologens (BBVs): 4,4'-o-BBV, 3,3'-o-BBV, 3,4'-o-BBV, 4,4'-o,m-BBV, 4,7'-o-PBBV, and pBoB, each coupled to the fluorophore 8-hydroxypyrene, 1,3,6-trisulfonic acid trisodium salt (HPTS). These probes were screened for their ability to discriminate between lactulose, l-rhamnose, 3-O-methyl-d-glucose, and xylose. Binding studies of sugar alcohols mannitol, sorbitol, erythritol, adonitol, arabitol, galactitol, and xylitol revealed that diols containing threo-1,2-diol units have higher affinity for BBVs relative diols containing erythro-1,2 units. Those containing both threo-1,2- and 1,3-syn diol","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 May","modification":"2025-04-26T18:38:45.638Z","creation":"2019-03-27T03:06:02Z"},"accession":"S-EPMC5747966","cross_references":{"pubmed":["27116118"],"doi":["10.1021/acs.analchem.6b00880"]}}