biostudies-literatureZacharias LGNational Institute of General Medical SciencesNIGMS NIH HHSCancer Prevention and Research Institute of Texas3624-3634https://www.ebi.ac.uk/biostudies/studies/S-EPMC5748158biostudies-literatureUnknown15(10)Aberrant glycosylation has been linked to many different cancer types. The blood-brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, and so on. However, in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Here we present a study of identifying and quantifying the glycosylation of six breast and brain cancer cell lines using hydrophilic interaction liquid chromatography (HILIC) and electrostatic repulsion liquid chromatography (ERLIC) enrichments and LC-MS/MS analysis. Qualitative and quantitative analyses of N-linked glycosylation were performed by both enrichment techniques for individual and complementary comparison. Potential cancer glycopeptide biomarkers were identified and confirmed by Journal of proteome researchHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells.PMC5748158R01 GM112490RP1306241 R01 GM112490Song EZhu RZacharias LGZhao JMirzaei PMechref YHartmann AKfalseHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells.Aberrant glycosylation has been linked to many different cancer types. The blood-brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, and so on. However, in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Here we present a study of identifying and quantifying the glycosylation of six breast and brain cancer cell lines using hydrophilic interaction liquid chromatography (HILIC) and electrostatic repulsion liquid chromatography (ERLIC) enrichments and LC-MS/MS analysis. Qualitative and quantitative analyses of N-linked glycosylation were performed by both enrichment techniques for individual and complementary comparison. Potential cancer glycopeptide biomarkers were identified and confirmed by 2016-01-01T00:00:00Z2016 Oct2025-04-26T18:36:32.621Z2019-03-27T03:06:01ZS-EPMC57481582753348510.1021/acs.jproteome.6b00429