{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hetta HF"],"funding":["NIDDK NIH HHS"],"pagination":["703-712"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5756491"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["65(8)"],"pubmed_abstract":["Extra-hepatic compartments might contribute to hepatitis C virus (HCV) persistence and extra-hepatic manifestations. Therefore, we investigated HCV infection in colonic tissue in patients with chronic hepatitis C (CHC) and its relationship with HCV pathogenesis. Colonic biopsies were collected from three groups with CHC infection: treatment naïve (TN; n=12), non-responders (NR; n=10) to anti-HCV therapy (pegylated interferon-α and ribavirin) and sustained virologic response (SVR; n=10) and from a fourth healthy control group (n=10). Liver biopsies were examined to assess inflammation and fibrosis. HCV infection and colonic T regulatory (Treg) frequency were detected by immunohistochemistry. HCV core and NS3 proteins were detected in B cells and macrophage/monocytes of 42 % and 25 % of TN and 50 % and 30 % of NR, respectively, but not in SVR or control group. The numbers of cells expressing HCV proteins were positively correlated with both HCV viral load and colonic Treg frequency. A significant negative correlation between HCV-expressing cells with both liver inflammation and fibrosis was identified. Our study provides evidence that HCV can infect B cells and macrophages of the colon. The correlations between HCV infection in colonic tissue and HCV viral load and liver pathology underline the significance of this extra-hepatic infection in HCV pathogenesis and response to therapy."],"journal":["Journal of medical microbiology"],"pubmed_title":["Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis."],"pmcid":["PMC5756491"],"funding_grant_id":["K24 DK070528","P30 DK078392"],"pubmed_authors":["Shata MTM","Hetta HF","Ahmed SH","Sherman KE","Mekky MA","Khalil NK","Daef EA","Nassar MI","El-Feky MA","Medhat A","Mohamed WA"],"additional_accession":[]},"is_claimable":false,"name":"Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis.","description":"Extra-hepatic compartments might contribute to hepatitis C virus (HCV) persistence and extra-hepatic manifestations. Therefore, we investigated HCV infection in colonic tissue in patients with chronic hepatitis C (CHC) and its relationship with HCV pathogenesis. Colonic biopsies were collected from three groups with CHC infection: treatment naïve (TN; n=12), non-responders (NR; n=10) to anti-HCV therapy (pegylated interferon-α and ribavirin) and sustained virologic response (SVR; n=10) and from a fourth healthy control group (n=10). Liver biopsies were examined to assess inflammation and fibrosis. HCV infection and colonic T regulatory (Treg) frequency were detected by immunohistochemistry. HCV core and NS3 proteins were detected in B cells and macrophage/monocytes of 42 % and 25 % of TN and 50 % and 30 % of NR, respectively, but not in SVR or control group. The numbers of cells expressing HCV proteins were positively correlated with both HCV viral load and colonic Treg frequency. A significant negative correlation between HCV-expressing cells with both liver inflammation and fibrosis was identified. Our study provides evidence that HCV can infect B cells and macrophages of the colon. The correlations between HCV infection in colonic tissue and HCV viral load and liver pathology underline the significance of this extra-hepatic infection in HCV pathogenesis and response to therapy.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Aug","modification":"2024-11-15T22:05:57.186Z","creation":"2019-03-27T00:14:38Z"},"accession":"S-EPMC5756491","cross_references":{"pubmed":["27166142"],"doi":["10.1099/jmm.0.000272"]}}