biostudies-literatureCitron YRIntramural NIH HHSHoward Hughes Medical InstituteNational Heart, Lung, and Blood InstituteNational Institutes of HealthDavid and Lucile Packard FoundationNIGMS NIH HHSNIH HHSe0190530https://www.ebi.ac.uk/biostudies/studies/S-EPMC5760045biostudies-literatureUnknown13(1)The centrosome serves as the main microtubule-organizing center in metazoan cells, yet despite its functional importance, little is known mechanistically about the structure and organizational principles that dictate protein organization in the centrosome. In particular, the protein-protein interactions that allow for the massive structural transition between the tightly organized interphase centrosome and the highly expanded matrix-like arrangement of the mitotic centrosome have been largely uncharacterized. Among the proteins that undergo a major transition is the Drosophila melanogaster protein centrosomin that contains a conserved carboxyl terminus motif, CM2. Recent crystal structures have shown this motif to be dimeric and capable of forming an intramolecular interaction with a centrPloS oneThe centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles.PMC5760045ZIA HL006104GM031627T32 GM008284DP2 OD008479DP2OD008479R01 GM0316271ZIAHL006104R35GM118099R35 GM118099Rusan NMCitron YRAgard DAFagerstrom CJKeszthelyi BHuang BKelly MJSfalseThe centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles.The centrosome serves as the main microtubule-organizing center in metazoan cells, yet despite its functional importance, little is known mechanistically about the structure and organizational principles that dictate protein organization in the centrosome. In particular, the protein-protein interactions that allow for the massive structural transition between the tightly organized interphase centrosome and the highly expanded matrix-like arrangement of the mitotic centrosome have been largely uncharacterized. Among the proteins that undergo a major transition is the Drosophila melanogaster protein centrosomin that contains a conserved carboxyl terminus motif, CM2. Recent crystal structures have shown this motif to be dimeric and capable of forming an intramolecular interaction with a centr2018-01-01T00:00:00Z20182026-04-07T18:54:56.937Z2019-03-26T23:49:42ZS-EPMC57600452931531910.1371/journal.pone.0190530