{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Fredericks GJ"],"pubmed_abstract":["Selenoprotein K (SELENOK) is a selenocysteine (Sec)-containing protein localized in the endoplasmic reticulum (ER) membrane where it interacts with the DHHC6 (where single letter symbols represent Asp-His-His-Cys amino acids) enzyme to promote protein acyl transferase (PAT) reactions. PAT reactions involve the DHHC enzymatic capture of palmitate via a thioester bond to cysteine (Cys) residues that form an unstable palmitoyl-DHHC intermediate, followed by transfer of palmitate to Cys residues of target proteins. How SELENOK facilitates this reaction has not been determined. Splenocyte microsomal preparations from wild-type mice versus SELENOK knockout mice were used to establish PAT assays and showed decreased PAT activity (~50%) under conditions of SELENOK deficiency. Using recombinant, so"],"journal":["Antioxidants (Basel, Switzerland)"],"pagination":["E4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5789314"],"repository":["biostudies-literature"],"pubmed_title":["Selenoprotein K Increases Efficiency of DHHC6 Catalyzed Protein Palmitoylation by Stabilizing the Acyl-DHHC6 Intermediate."],"pmcid":["PMC5789314"],"pubmed_authors":["Hoffmann FW","Hoffmann PR","Rozovsky S","Hondal RJ","Urschitz J","Fredericks GJ"],"additional_accession":[]},"is_claimable":false,"name":"Selenoprotein K Increases Efficiency of DHHC6 Catalyzed Protein Palmitoylation by Stabilizing the Acyl-DHHC6 Intermediate.","description":"Selenoprotein K (SELENOK) is a selenocysteine (Sec)-containing protein localized in the endoplasmic reticulum (ER) membrane where it interacts with the DHHC6 (where single letter symbols represent Asp-His-His-Cys amino acids) enzyme to promote protein acyl transferase (PAT) reactions. PAT reactions involve the DHHC enzymatic capture of palmitate via a thioester bond to cysteine (Cys) residues that form an unstable palmitoyl-DHHC intermediate, followed by transfer of palmitate to Cys residues of target proteins. How SELENOK facilitates this reaction has not been determined. Splenocyte microsomal preparations from wild-type mice versus SELENOK knockout mice were used to establish PAT assays and showed decreased PAT activity (~50%) under conditions of SELENOK deficiency. Using recombinant, so","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Dec","modification":"2025-04-22T21:31:08.295Z","creation":"2019-03-26T23:00:15Z"},"accession":"S-EPMC5789314","cross_references":{"pubmed":["29286308"],"doi":["10.3390/antiox7010004"]}}