{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kornilov R"],"funding":["Biotieteiden ja Ympäristön Tutkimuksen Toimikunta","Helsingin ja Uudenmaan Sairaanhoitopiiri","Tekes","Private donation/University of Helsinki"],"pagination":["1422674"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5795649"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments. However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses. Therefore, a prior elimination of EVs from FBS is crucial. However, the current methods of EV depletion by ultracentrifugation are cumbersome and the commercial alternatives expensive. In this study, our aim was to develop a protocol to completely deplete EVs from FBS, which may have wide applicability in cell-culture applications. We investigated different EV-depleted FBS prepared by our novel ultrafiltration-based protocol, by conventionally used overnight ultracentrifugation, or commercially"],"journal":["Journal of extracellular vesicles"],"pubmed_title":["Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum."],"pmcid":["PMC5795649"],"funding_grant_id":["287089","Y1014SUL05","5773/31/16","WBS73714301","3986/31/2013","40294/11","TYH2016130"],"pubmed_authors":["Hiidenmaa H","Puhka M","Siljander P","Peltoniemi H","Kornilov R","Mannerstrom B","Seppanen-Kaijansinkko R","Kaur S"],"additional_accession":[]},"is_claimable":false,"name":"Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum.","description":"Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments. However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses. Therefore, a prior elimination of EVs from FBS is crucial. However, the current methods of EV depletion by ultracentrifugation are cumbersome and the commercial alternatives expensive. In this study, our aim was to develop a protocol to completely deplete EVs from FBS, which may have wide applicability in cell-culture applications. We investigated different EV-depleted FBS prepared by our novel ultrafiltration-based protocol, by conventionally used overnight ultracentrifugation, or commercially","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-27T04:34:51.038Z","creation":"2019-03-26T23:00:55Z"},"accession":"S-EPMC5795649","cross_references":{"pubmed":["29410778"],"doi":["10.1080/20013078.2017.1422674"]}}