{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["19(1)"],"submitter":["Stephan A"],"pubmed_abstract":["Colicins are natural non-antibiotic bacterial proteins with a narrow spectrum but an extremely high antibacterial activity. These proteins are promising food additives for the control of major pathogenic Shiga toxin-producing E. coli serovars in meats and produce. In the USA, colicins produced in edible plants such as spinach and leafy beets have already been accepted by the U. S. Food and Drug Administration (FDA) and U. S. Department of Agriculture (USDA) as food-processing antibacterials through the GRAS (generally recognized as safe) regulatory review process. Nicotiana benthamiana, a wild relative of tobacco, N. tabacum, has become the preferred production host plant for manufacturing recombinant proteins-including biopharmaceuticals, vaccines, and biomaterials-but the purification pr"],"journal":["International journal of molecular sciences"],"pagination":["E95"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5796045"],"repository":["biostudies-literature"],"pubmed_title":["Simple Purification of Nicotiana benthamiana-Produced Recombinant Colicins: High-Yield Recovery of Purified Proteins with Minimum Alkaloid Content Supports the Suitability of the Host for Manufacturing Food Additives."],"pmcid":["PMC5796045"],"pubmed_authors":["Buchholz M","Rosche F","Hahn-Lobmann S","Stephan A","Giritch A","Gleba Y"],"additional_accession":[]},"is_claimable":false,"name":"Simple Purification of Nicotiana benthamiana-Produced Recombinant Colicins: High-Yield Recovery of Purified Proteins with Minimum Alkaloid Content Supports the Suitability of the Host for Manufacturing Food Additives.","description":"Colicins are natural non-antibiotic bacterial proteins with a narrow spectrum but an extremely high antibacterial activity. These proteins are promising food additives for the control of major pathogenic Shiga toxin-producing E. coli serovars in meats and produce. In the USA, colicins produced in edible plants such as spinach and leafy beets have already been accepted by the U. S. Food and Drug Administration (FDA) and U. S. Department of Agriculture (USDA) as food-processing antibacterials through the GRAS (generally recognized as safe) regulatory review process. Nicotiana benthamiana, a wild relative of tobacco, N. tabacum, has become the preferred production host plant for manufacturing recombinant proteins-including biopharmaceuticals, vaccines, and biomaterials-but the purification pr","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Dec","modification":"2025-06-01T03:28:04.95Z","creation":"2025-06-01T03:28:04.95Z"},"accession":"S-EPMC5796045","cross_references":{"pubmed":["29286298"],"doi":["10.3390/ijms19010095"]}}