{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang L"],"funding":["Natural Science Foundation of Shanghai"],"pagination":["2790864"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5804362"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2017"],"pubmed_abstract":["<h4>Background</h4>Nonalcoholic fatty liver disease (NAFLD) has become a leading cause of liver transplantation. <i>Lingguizhugan</i> decoction (LGZG), a classical Chinese herbal formula, has beneficial effects on NAFLD animal models. Our study examined the impact of LGZG on hepatic global transcriptome of high-fat-diet-induced NAFLD rats.<h4>Methods</h4>Three groups of Wistar rats were included: normal, NAFLD model, and LGZG-treated NAFLD groups. Four weeks for the treatment, liver tissues were harvested for RNA sequencing. Differentially expressed genes (DEGs) and enriched pathways were detected on hepatic global transcriptome profile. Real-time PCR validated the regulatory patterns of LGZG on NAFLD rats.<h4>Results</h4>DEGs between the NAFLD model and normal groups indicated the elevated peroxisome proliferator-activated receptor (PPAR) and hedgehog signaling pathways in NAFLD rats. In bile secretion pathway, genes involved in cholesterol secretion were activated by LGZG treatment. Increased expression of antioxidant <i>OSIGN1</i> and decreased expression of genes (<i>AHR</i>, <i>IRF2BP2</i>, and <i>RASGEF1B</i>) that induce oxidative stress and inflammation were observed in NAFLD rats treated with LGZG. The regulatory patterns of LGZG treatment on these oxidative stress-related genes were confirmed by real-time PCR.<h4>Conclusion</h4>Our study revealed a \"two-hits-targeting\" mechanism of LGZG in the treatment for NAFLD: alleviating oxidative stress and activating cholesterol secretion."],"journal":["International journal of genomics"],"pubmed_title":["<i>Lingguizhugan</i> Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion."],"pmcid":["PMC5804362"],"funding_grant_id":["15ZR1441900","81620108030"],"pubmed_authors":["Nugent CA","Yang L","Lin W","Zheng P","Song H","Liu T","Hao S"],"additional_accession":[]},"is_claimable":false,"name":"<i>Lingguizhugan</i> Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion.","description":"<h4>Background</h4>Nonalcoholic fatty liver disease (NAFLD) has become a leading cause of liver transplantation. <i>Lingguizhugan</i> decoction (LGZG), a classical Chinese herbal formula, has beneficial effects on NAFLD animal models. Our study examined the impact of LGZG on hepatic global transcriptome of high-fat-diet-induced NAFLD rats.<h4>Methods</h4>Three groups of Wistar rats were included: normal, NAFLD model, and LGZG-treated NAFLD groups. Four weeks for the treatment, liver tissues were harvested for RNA sequencing. Differentially expressed genes (DEGs) and enriched pathways were detected on hepatic global transcriptome profile. Real-time PCR validated the regulatory patterns of LGZG on NAFLD rats.<h4>Results</h4>DEGs between the NAFLD model and normal groups indicated the elevated peroxisome proliferator-activated receptor (PPAR) and hedgehog signaling pathways in NAFLD rats. In bile secretion pathway, genes involved in cholesterol secretion were activated by LGZG treatment. Increased expression of antioxidant <i>OSIGN1</i> and decreased expression of genes (<i>AHR</i>, <i>IRF2BP2</i>, and <i>RASGEF1B</i>) that induce oxidative stress and inflammation were observed in NAFLD rats treated with LGZG. The regulatory patterns of LGZG treatment on these oxidative stress-related genes were confirmed by real-time PCR.<h4>Conclusion</h4>Our study revealed a \"two-hits-targeting\" mechanism of LGZG in the treatment for NAFLD: alleviating oxidative stress and activating cholesterol secretion.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2024-12-04T06:00:56.858Z","creation":"2019-03-26T23:03:19Z"},"accession":"S-EPMC5804362","cross_references":{"pubmed":["29464180"],"doi":["10.1155/2017/2790864"]}}