biostudies-literatureUnknown6(2)Sun SCurrent multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.Chemical science930-934https://www.ebi.ac.uk/biostudies/studies/S-EPMC5811145biostudies-literatureMultiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures.PMC5811145Zhang FZhu JSun SYao HfalseMultiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures.Current multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.2015-01-01T00:00:00Z2015 Feb2025-04-04T19:45:37.29Z2019-03-26T23:22:33ZS-EPMC58111452956017910.1039/c4sc02696a