biostudies-literatureUnknown13Weller MGThe quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria.Analytical chemistry insights1177390118757462https://www.ebi.ac.uk/biostudies/studies/S-EPMC5813849biostudies-literatureTen Basic Rules of Antibody Validation.PMC5813849Weller MGfalseTen Basic Rules of Antibody Validation.The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria.2018-01-01T00:00:00Z20182020-11-20T08:13:31Z2019-03-26T23:03:26ZS-EPMC58138492946756910.1177/1177390118757462