biostudies-literatureGeiselhart SAustrian Science Fund FWF217-224https://www.ebi.ac.uk/biostudies/studies/S-EPMC5814722biostudies-literatureUnknown48(2)<h4>Background</h4>Buckwheat (Fagopyrum esculentum) has become increasingly popular as a healthy food in Europe. However, for sensitized individuals, consumption can cause anaphylactic reactions. The aim of this study was to identify individual well-characterized buckwheat allergens for component-resolved diagnosis.<h4>Methods</h4>Patients were selected by positive skin prick test to buckwheat and divided into two groups: (1) sensitized to buckwheat without clinical symptoms and (2) buckwheat allergy. Buckwheat proteins were extracted from raw buckwheat seeds, purified applying a combination of protein precipitation and chromatographic methods, and analyzed by IgE immunoblotting and ELISA.<h4>Results</h4>Buckwheat-allergic patients had a significantly larger median skin prick test weal diaClinical and experimental allergy : journal of the British Society for Allergy and Clinical ImmunologyConcomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy.PMC5814722SFB F4603W1248Geiselhart SNagl CDubiela PRadauer CPedersen ACBublin MBindslev-Jensen CHoffmann-Sommergruber KMortz CGfalseConcomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy.<h4>Background</h4>Buckwheat (Fagopyrum esculentum) has become increasingly popular as a healthy food in Europe. However, for sensitized individuals, consumption can cause anaphylactic reactions. The aim of this study was to identify individual well-characterized buckwheat allergens for component-resolved diagnosis.<h4>Methods</h4>Patients were selected by positive skin prick test to buckwheat and divided into two groups: (1) sensitized to buckwheat without clinical symptoms and (2) buckwheat allergy. Buckwheat proteins were extracted from raw buckwheat seeds, purified applying a combination of protein precipitation and chromatographic methods, and analyzed by IgE immunoblotting and ELISA.<h4>Results</h4>Buckwheat-allergic patients had a significantly larger median skin prick test weal dia2018-01-01T00:00:00Z2018 Feb2026-04-28T23:12:05.924Z2026-04-13T03:06:57.898ZS-EPMC58147222917191210.1111/cea.13068