{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["46(3)"],"submitter":["Yang F"],"pubmed_abstract":["Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi-library screening can be accomplished in a single pool without any individual treatment. This system is based on the phiC31 integrase-mediated integration between bait and prey plasmids. The integrated fragments were digested by MmeI and subjected to deep sequencing to decode the interaction matrix"],"journal":["Nucleic acids research"],"pagination":["e17"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5815087"],"repository":["biostudies-literature"],"pubmed_title":["Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system."],"pmcid":["PMC5815087"],"pubmed_authors":["Zhou M","Habib Z","Cao G","Wang D","Fu Z","Zhong W","Han Y","Yao Q","Chen H","Xiao S","Yang F","Rahman K","Zhu C","Lin D","Lei Y","Tao R","Chen X","Xu W","Tyagi R","Wu X","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system.","description":"Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi-library screening can be accomplished in a single pool without any individual treatment. This system is based on the phiC31 integrase-mediated integration between bait and prey plasmids. The integrated fragments were digested by MmeI and subjected to deep sequencing to decode the interaction matrix","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Feb","modification":"2026-04-28T22:42:00.987Z","creation":"2019-03-26T23:03:30Z"},"accession":"S-EPMC5815087","cross_references":{"pubmed":["29165646"],"doi":["10.1093/nar/gkx1173"]}}