biostudies-literatureBullman SNCATS NIH HHSClark Family Fund for GI Cancer ResearchHoward Hughes Medical InstituteProject P Fund for Colorectal Cancer ResearchNational Institutes of HealthHale Family Center for Pancreatic CancerPrevent Cancer FoundationCellex Private FoundationChambers Family Fund for Colorectal Cancer ResearchDF/HCC GI SPORE P50Banco Bilbao Vizcaya Argentaria FoundationStand-up-to-CancerNational Institute of HealthTeam Perry FundNCI NIH HHSAmerican Cancer Society Research Professorship1443-1448https://www.ebi.ac.uk/biostudies/studies/S-EPMC5823247biostudies-literatureUnknown358(6369)Colorectal cancers comprise a complex mixture of malignant cells, nontransformed cells, and microorganisms. <i>Fusobacterium nucleatum</i> is among the most prevalent bacterial species in colorectal cancer tissues. Here we show that colonization of human colorectal cancers with <i>Fusobacterium</i> and its associated microbiome-including <i>Bacteroides</i>, <i>Selenomonas</i>, and <i>Prevotella</i> species-is maintained in distal metastases, demonstrating microbiome stability between paired primary and metastatic tumors. In situ hybridization analysis revealed that <i>Fusobacterium</i> is predominantly associated with cancer cells in the metastatic lesions. Mouse xenografts of human primary colorectal adenocarcinomas were found to retain viable <i>Fusobacterium</i> and its associated microScience (New York, N.Y.)Analysis of <i>Fusobacterium</i> persistence and antibiotic response in colorectal cancer.PMC5823247CA127003R35 CA197735CA148894R35 CA197568UL1 TR001102Figdor Family FellowshipUL1 TR001863CA169141K07 CA148894CA205406R01 CA118553CA197735R01 CA205406CA118553CA197568P50 CA127003R01 CA169141Fasani RElez ESicinska EMulet NPedamallu CSHuang KNuciforo PHagan TRamon Y Cajal SBullman SCai DRamachandran AAguirre AJHahn WCNg KClancy TEWalker MSerna GTabernero JLandolfi SOgino SChipashvili ONeuberg DNelson TGuevara FZhang XFuchs CSMeyerson MDiosdado BfalseAnalysis of <i>Fusobacterium</i> persistence and antibiotic response in colorectal cancer.Colorectal cancers comprise a complex mixture of malignant cells, nontransformed cells, and microorganisms. <i>Fusobacterium nucleatum</i> is among the most prevalent bacterial species in colorectal cancer tissues. Here we show that colonization of human colorectal cancers with <i>Fusobacterium</i> and its associated microbiome-including <i>Bacteroides</i>, <i>Selenomonas</i>, and <i>Prevotella</i> species-is maintained in distal metastases, demonstrating microbiome stability between paired primary and metastatic tumors. In situ hybridization analysis revealed that <i>Fusobacterium</i> is predominantly associated with cancer cells in the metastatic lesions. Mouse xenografts of human primary colorectal adenocarcinomas were found to retain viable <i>Fusobacterium</i> and its associated micro2017-01-01T00:00:00Z2017 Dec2026-05-05T03:44:03.249Z2019-03-27T00:11:24ZS-EPMC58232472917028010.1126/science.aal5240