{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["185(3)"],"submitter":["Wang R"],"pubmed_abstract":["An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several ?-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free ?-agonists compete for the binding sites. This enables rapid screening for the 7 ?-agonists in a single run that takes about 8 min. Detection limits for the seven ?-agonists are <50 pg g-1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for ?-agonists, and the results compare well to those obtained by HPLC-MS. Graphical abstractSchematic presentation of an ultra sensitive fluorescent nanoparticle based paper based assay for rapid detection of multi ?-agonists in pork tissue."],"journal":["Mikrochimica acta"],"pagination":["191"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5823949"],"repository":["biostudies-literature"],"pubmed_title":["A paper-based competitive lateral flow immunoassay for multi ?-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles."],"pmcid":["PMC5823949"],"pubmed_authors":["Zhang W","Wang P","Wang R","Su X"],"additional_accession":[]},"is_claimable":false,"name":"A paper-based competitive lateral flow immunoassay for multi ?-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles.","description":"An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several ?-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free ?-agonists compete for the binding sites. This enables rapid screening for the 7 ?-agonists in a single run that takes about 8 min. Detection limits for the seven ?-agonists are <50 pg g-1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for ?-agonists, and the results compare well to those obtained by HPLC-MS. Graphical abstractSchematic presentation of an ultra sensitive fluorescent nanoparticle based paper based assay for rapid detection of multi ?-agonists in pork tissue.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2020-10-29T10:12:34Z","creation":"2019-03-26T23:05:37Z"},"accession":"S-EPMC5823949","cross_references":{"pubmed":["29503465"],"doi":["10.1007/s00604-018-2730-9"]}}