biostudies-literature00600Protter DSWT32Howard Hughes Medical InstituteWelch FoundationNIHNIGMS NIH HHS1401-1412https://www.ebi.ac.uk/biostudies/studies/S-EPMC5824733biostudies-literatureUnknown22(6)Eukaryotic cells contain large RNA-protein assemblies referred to as RNP granules, whose assembly is promoted by both traditional protein interactions and intrinsically disordered protein domains. Using RNP granules as an example, we provide evidence for an assembly mechanism of large cellular structures wherein specific protein-protein or protein-RNA interactions act together with promiscuous interactions of intrinsically disordered regions (IDRs). This synergistic assembly mechanism illuminates RNP granule assembly and explains why many components of RNP granules, and other large dynamic assemblies, contain IDRs linked to specific protein-protein or protein-RNA interaction modules. We suggest assemblies based on combinations of specific interactions and promiscuous IDRs are common features of eukaryotic cells.Cell reportsIntrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly.PMC5824733GM063235R01 GM045443T32 GM065103T32 GM142607HCIA grantGM045443R01-GM56322I-1544T32 GM008759Protter DSWRao BSVan Treeck BMizoue LLin YRosen MKParker R60falseIntrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly.Eukaryotic cells contain large RNA-protein assemblies referred to as RNP granules, whose assembly is promoted by both traditional protein interactions and intrinsically disordered protein domains. Using RNP granules as an example, we provide evidence for an assembly mechanism of large cellular structures wherein specific protein-protein or protein-RNA interactions act together with promiscuous interactions of intrinsically disordered regions (IDRs). This synergistic assembly mechanism illuminates RNP granule assembly and explains why many components of RNP granules, and other large dynamic assemblies, contain IDRs linked to specific protein-protein or protein-RNA interaction modules. We suggest assemblies based on combinations of specific interactions and promiscuous IDRs are common features of eukaryotic cells.2018-01-01T00:00:00Z2018 Feb2024-12-04T06:02:34.098Z2019-03-26T23:03:53ZS-EPMC58247332942549710.1016/j.celrep.2018.01.036