{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jorge PH"],"funding":["Conselho Nacional de Desenvolvimento Científico e Tecnológico","Fundação de Amparo à Pesquisa do Estado de São Paulo"],"pagination":["46"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5827183"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs. Of this total, 2,568 contigs had similarity in the non-redundant (nr) protein database (Genbank) and 2,075 sequences were characterized in the categories of Gene Ontology (GO). After the validat"],"journal":["Frontiers in genetics"],"pubmed_title":["Genetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing."],"pmcid":["PMC5827183"],"funding_grant_id":["305916/2015-7","2014/05732-2","2014/03772-7","446779/2014-8"],"pubmed_authors":["Jorge PH","Mendes NJ","Hashimoto DT","Mastrochirico-Filho VA","Hata ME","Porto-Foresti F","de Freitas MV","Vera M","Ariede RB"],"additional_accession":[]},"is_claimable":false,"name":"Genetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing.","description":"The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs. Of this total, 2,568 contigs had similarity in the non-redundant (nr) protein database (Genbank) and 2,075 sequences were characterized in the categories of Gene Ontology (GO). After the validat","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-22T04:25:49.07Z","creation":"2019-06-06T19:01:14Z"},"accession":"S-EPMC5827183","cross_references":{"pubmed":["29520294"],"doi":["10.3389/fgene.2018.00046"]}}