biostudies-literatureJorge PHConselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de São Paulo46https://www.ebi.ac.uk/biostudies/studies/S-EPMC5827183biostudies-literatureUnknown9The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs. Of this total, 2,568 contigs had similarity in the non-redundant (nr) protein database (Genbank) and 2,075 sequences were characterized in the categories of Gene Ontology (GO). After the validatFrontiers in geneticsGenetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing.PMC5827183305916/2015-72014/05732-22014/03772-7446779/2014-8Jorge PHMendes NJHashimoto DTMastrochirico-Filho VAHata MEPorto-Foresti Fde Freitas MVVera MAriede RBfalseGenetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing.The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs. Of this total, 2,568 contigs had similarity in the non-redundant (nr) protein database (Genbank) and 2,075 sequences were characterized in the categories of Gene Ontology (GO). After the validat2018-01-01T00:00:00Z20182025-04-22T04:25:49.07Z2019-06-06T19:01:14ZS-EPMC58271832952029410.3389/fgene.2018.00046