biostudies-literatureGu WJapan Agency for Medical Research and DevelopmentMinistry of Education, Culture, Sports, Science and Technologybio032318https://www.ebi.ac.uk/biostudies/studies/S-EPMC5829515biostudies-literatureUnknown7(1)Spontaneous testicular teratoma develops from primordial germ cells (PGCs) in embryos; however, the molecular mechanisms underlying teratoma formation are not fully understood. Mutation of the dead-end 1 (<i>Dnd1</i>) gene, which encodes an RNA-binding protein, drastically enhances teratoma formation in the 129/Sv mouse strain. To elucidate the mechanism of <i>Dnd1</i> mutation-induced teratoma formation, we focused on histone H3 lysine 27 (H3K27) trimethylation (me3), and found that the levels of H3K27me3 and its responsible methyltransferase, enhancer of zeste homolog 2 (Ezh2), were decreased in the teratoma-forming cells of <i>Dnd1</i> mutant embryos. We also showed that Dnd1 suppressed miR-26a-mediated inhibition of Ezh2 expression, and that Dnd1 deficiency resulted in decreased H3K27mBiology openDnd1-mediated epigenetic control of teratoma formation in mouse.PMC5829515JP17gm0510017h25114003Matsui YMochizuki KGu WOtsuka KHamada RTakehara AfalseDnd1-mediated epigenetic control of teratoma formation in mouse.Spontaneous testicular teratoma develops from primordial germ cells (PGCs) in embryos; however, the molecular mechanisms underlying teratoma formation are not fully understood. Mutation of the dead-end 1 (<i>Dnd1</i>) gene, which encodes an RNA-binding protein, drastically enhances teratoma formation in the 129/Sv mouse strain. To elucidate the mechanism of <i>Dnd1</i> mutation-induced teratoma formation, we focused on histone H3 lysine 27 (H3K27) trimethylation (me3), and found that the levels of H3K27me3 and its responsible methyltransferase, enhancer of zeste homolog 2 (Ezh2), were decreased in the teratoma-forming cells of <i>Dnd1</i> mutant embryos. We also showed that Dnd1 suppressed miR-26a-mediated inhibition of Ezh2 expression, and that Dnd1 deficiency resulted in decreased H3K27m2018-01-01T00:00:00Z2018 Jan2026-06-18T04:53:19.15Z2019-03-26T23:04:28ZS-EPMC58295152937870210.1242/bio.032318