{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(1)"],"submitter":["Chang LL"],"pubmed_abstract":["Tissue stroma is known to be important in regulating Hp-mediated inflammation, but its interaction with Hp and dendritic cells (DCs) remains to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs was investigated. Primary GSCs from cancerous and adjacent normal tissues were generated from gastric cancer patients, and monocyte-derived DCs were obtained from healthy individuals. Levels of cytokines and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) were measured by ELISA, and C-type lectin expression in GSCs was assessed by flow cytometry and immunohistochemistry. In a trans-well co-culture system, significantly upregulated DC-derived IL-23 expression was found when DCs were co-cultured with Hp-infected GSCs (Hp-GSCs). Fu"],"journal":["Scientific reports"],"pagination":["3821"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5830506"],"repository":["biostudies-literature"],"pubmed_title":["Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases."],"pmcid":["PMC5830506"],"pubmed_authors":["Hsu WH","Lin MH","Liu CJ","Lin CC","Wang CJ","Kuo FC","Weng BC","Chang LL","Kao MC","Huang SK","Chou CC","Lin CH","Wu DC","Kuo CH"],"additional_accession":[]},"is_claimable":false,"name":"Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases.","description":"Tissue stroma is known to be important in regulating Hp-mediated inflammation, but its interaction with Hp and dendritic cells (DCs) remains to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs was investigated. Primary GSCs from cancerous and adjacent normal tissues were generated from gastric cancer patients, and monocyte-derived DCs were obtained from healthy individuals. Levels of cytokines and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) were measured by ELISA, and C-type lectin expression in GSCs was assessed by flow cytometry and immunohistochemistry. In a trans-well co-culture system, significantly upregulated DC-derived IL-23 expression was found when DCs were co-cultured with Hp-infected GSCs (Hp-GSCs). Fu","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Feb","modification":"2025-04-19T00:09:14.09Z","creation":"2019-03-26T23:05:40Z"},"accession":"S-EPMC5830506","cross_references":{"pubmed":["29491476"],"doi":["10.1038/s41598-018-20957-2"]}}