biostudies-literatureUnknown6(2)Sasikala M<h4>Objectives</h4>Altered immune homeostasis and involvement of T cells has been reported in chronic pancreatitis (CP). We evaluated the role of <i>Bach2</i> (BTB and CNC homology basic leucine zipper transcription factor 2), a key regulator of immune homeostasis in the chronicity of CP.<h4>Methods</h4>Expression of <i>Bach2</i> and T-cell transcription factors, enumeration of BACH2+/CD4+ T-lymphocytes were performed by qRT-PCR and flow cytometry respectively. <i>Bach2</i>silenced human CD4+ T-lymphocytes were exposed to CP tissue extract to assess T-cell lineage commitment. Aryl hydrocarbon receptor (<i>Ahr</i>) and Deubiquitinase enzyme A (DUBA/<i>OTUD5gene</i>) were evaluated as markers of persistent Th17 cell differentiation. <i>Bach2</i> gene (exons) was sequenced to identify risk vaUnited European gastroenterology journal272-282https://www.ebi.ac.uk/biostudies/studies/S-EPMC5833222biostudies-literatureBach2 repression mediates Th17 cell induced inflammation and associates with clinical features of advanced disease in chronic pancreatitis.PMC5833222Murali Manohar KGhosh SAslam MPavan Kumar PPradeep RTalukdar RReddy DNDeshpande NRao GVRavikanth VVSingh SSasikala MfalseBach2 repression mediates Th17 cell induced inflammation and associates with clinical features of advanced disease in chronic pancreatitis.<h4>Objectives</h4>Altered immune homeostasis and involvement of T cells has been reported in chronic pancreatitis (CP). We evaluated the role of <i>Bach2</i> (BTB and CNC homology basic leucine zipper transcription factor 2), a key regulator of immune homeostasis in the chronicity of CP.<h4>Methods</h4>Expression of <i>Bach2</i> and T-cell transcription factors, enumeration of BACH2+/CD4+ T-lymphocytes were performed by qRT-PCR and flow cytometry respectively. <i>Bach2</i>silenced human CD4+ T-lymphocytes were exposed to CP tissue extract to assess T-cell lineage commitment. Aryl hydrocarbon receptor (<i>Ahr</i>) and Deubiquitinase enzyme A (DUBA/<i>OTUD5gene</i>) were evaluated as markers of persistent Th17 cell differentiation. <i>Bach2</i> gene (exons) was sequenced to identify risk va2018-01-01T00:00:00Z2018 Mar2026-05-05T03:46:19.574Z2026-04-07T21:22:01.961ZS-EPMC58332222951155710.1177/2050640617716596