biostudies-literatureUnknown2018Zhao ZGerman Ministry of Education and Research<h4>Background</h4>Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. However, the ion channel expression and functions as well as regulations in hiPSC-CMs still need to be fully characterized.<h4>Methods</h4>Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors. qPCR and patch clamp techniques were used for the study.<h4>Results</h4>In addition to the reported ion channels, I_Na, I_Ca-L, I_Ca-T, I_f, I_NCX, I_K1, I_to, I_Kr, I_Ks I_KATP, I_K-pH, I_SK1-3, and I_SK4, we detected both the expression and currents of ACh-activated (KACh) and Na⁺-activated (KNa) K⁺, volume-regulated and calcium-activated (Cl-Ca) Cl^-, and TRPV channels. All the detected ion currents except I_K1, I_KACh, I_SK, I_KNa, and TRPV1 currents contribute to AP duration. Isoprenaline increased I_Ca-L, I_f, and I_Ks but reduced I_Na and I_NCX, without an effect on I_to, I_K1, I_SK1-3, I_KATP, I_Kr, I_SK4, I_KNa, I_Cl-Ca, and I_TRPV1. Carbachol alone showed no effect on the tested ion channel currents.<h4>Conclusion</h4>Our data demonstrate that most ion channels, which are present in healthy or diseased cardiomyocytes, exist in hiPSC-CMs. Some of them contribute to action potential performance and are regulated by adrenergic stimulation.Stem cells international6067096https://www.ebi.ac.uk/biostudies/studies/S-EPMC5835237biostudies-literatureIon Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.PMC5835237Yucel GTiburcy MUtikal JZimmermann WHBuljubasic FCyganek LZhou XBZhao ZBorggrefe MLi XAkin IEl-Battrawy IWieland TLang SLan HSattler KfalseIon Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.<h4>Background</h4>Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. However, the ion channel expression and functions as well as regulations in hiPSC-CMs still need to be fully characterized.<h4>Methods</h4>Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors. qPCR and patch clamp techniques were used for the study.<h4>Results</h4>In addition to the reported ion channels, I_Na, I_Ca-L, I_Ca-T, I_f, I_NCX, I_K1, I_to, I_Kr, I_Ks I_KATP, I_K-pH, I_SK1-3, and I_SK4, we detected both the expression and currents of ACh-activated (KACh) and Na⁺-activated (KNa) K⁺, volume-regulated and calcium-activated (Cl-Ca) Cl^-, and TRPV channels. All the detected ion currents except I_K1, I_KACh, I_SK, I_KNa, and TRPV1 currents contribute to AP duration. Isoprenaline increased I_Ca-L, I_f, and I_Ks but reduced I_Na and I_NCX, without an effect on I_to, I_K1, I_SK1-3, I_KATP, I_Kr, I_SK4, I_KNa, I_Cl-Ca, and I_TRPV1. Carbachol alone showed no effect on the tested ion channel currents.<h4>Conclusion</h4>Our data demonstrate that most ion channels, which are present in healthy or diseased cardiomyocytes, exist in hiPSC-CMs. Some of them contribute to action potential performance and are regulated by adrenergic stimulation.2018-01-01T00:00:00Z20182026-05-05T23:19:26.139Z2019-03-26T23:17:51ZS-EPMC58352372953577310.1155/2018/6067096